ShanghaiTech University Knowledge Management System
| Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling | |
| 2020-01 | |
| 发表期刊 | ACS CHEMICAL BIOLOGY (IF:3.5[JCR-2023],3.9[5-Year]) |
| ISSN | 1554-8929 |
| EISSN | 1554-8937 |
| 卷号 | 15期号:1页码:39-43 |
| 发表状态 | 已发表 |
| DOI | 10.1021/acschembio.9b00842 |
| 摘要 | Proximity labeling has been used to study protein-protein interactions and can also be used as a protein labeling tool. We developed a novel 14-amino acid peptide substrate for the proximity-labeling enzyme PafA. The N terminus of the peptide can be modified with biotin or fluorophores, which allows various chemical moieties to be ligated to the target protein. We used PafA-mediated peptide labeling to label antibodies with a biotin tag without affecting the antigen binding capacity of the antibody. Similar strategies can be used for other types of protein labeling in the future. |
| 收录类别 | SCI ; SCIE |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31570767] ; National Natural Science Foundation of China[31870774] ; National Natural Science Foundation of China[31922038] ; National Natural Science Foundation of China[21704064] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000508471000005 |
| 出版者 | AMER CHEMICAL SOC |
| WOS关键词 | UBIQUITIN-LIKE PROTEIN ; LIGASE ; PEROXIDASE ; COMPLEX ; CELLS |
| 原始文献类型 | Article |
| 引用统计 | 正在获取...
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| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/105311 |
| 专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_庄敏组 iHuman研究所 iHuman研究所_特聘教授组_Andrej Sali组 iHuman研究所_PI研究组_赵素文组 生命科学与技术学院_硕士生 |
| 通讯作者 | Zhuang, Min |
| 作者单位 | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China |
| 第一作者单位 | 生命科学与技术学院 |
| 通讯作者单位 | 生命科学与技术学院 |
| 第一作者的第一单位 | 生命科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Sun, Weiping,Huo, Yinbo,Mei, Yuxuan,et al. Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling[J]. ACS CHEMICAL BIOLOGY,2020,15(1):39-43. |
| APA | Sun, Weiping,Huo, Yinbo,Mei, Yuxuan,Zhou, Qingtong,Zhao, Suwen,&Zhuang, Min.(2020).Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.ACS CHEMICAL BIOLOGY,15(1),39-43. |
| MLA | Sun, Weiping,et al."Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling".ACS CHEMICAL BIOLOGY 15.1(2020):39-43. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
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