Cryo-EM structure of Nipah virus RNA polymerase complex

doi:10.1126/sciadv.adr7116

KMS > iHuman研究所

	Cryo-EM structure of Nipah virus RNA polymerase complex
	Yiru Wang1,3 ; Lixia Zhao1 ; Yi Zhang1 ; Yuhan Wang2,3 ; Jiao Tang 5; Simiao Liu 6; Huihan Gao1,3 ; Xiaoxiao Zhang2 ; Luca Zinzula2 ; Roger D. Kornberg1,4 ; Heqiao Zhang1
	2024-12-11
发表期刊	SCIENCE ADVANCES (IF:11.7[JCR-2023],13.7[5-Year])
ISSN	2375-2548
卷号	10 期号:50
发表状态	已发表
DOI	10.1126/sciadv.adr7116
摘要	Nipah virus, a member of the Paramyxoviridae family, is a highly pathogenic nonsegmented, negative-sense RNA virus (nsNSV) which causes severe neurological and respiratory illnesses in humans. There are no available drugs or vaccines to combat this virus. A complex of large polymerase protein (L) and phosphoprotein (P) of Nipah virus supports replication and transcription and affords a target for antiviral drug development. Structural information required for drug development is lacking. Here we report the 2.9-angstrom cryo-electron microscopy structure of the Nipah virus polymerase-phosphoprotein complex. The structure identifies conserved amino acids likely important for recognition of template RNA by nsNSVs and reveals the locations of mutation-prone sites among Nipah virus strains, which may facilitate the development of therapeutic agents against Nipah virus by targeting regions unaffected by these mutation sites.
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收录类别	SCI
语种	英语
资助项目	Shanxi Key Laboratory of Protein Structure Determination[202104010910006]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001375631700013
出版者	AMER ASSOC ADVANCEMENT SCIENCE
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/449060
专题	iHuman研究所生命科学与技术学院_PI研究组_黄鹏羽组免疫化学研究所免疫化学研究所_特聘教授组_结构生物化学实验室生命科学与技术学院_硕士生生命科学与技术学院_博士生 iHuman研究所_特聘教授组_Wolfgang Baumeister组
共同第一作者	Lixia Zhao; Yi Zhang; Yuhan Wang
通讯作者	Xiaoxiao Zhang; Luca Zinzula; Roger D. Kornberg; Heqiao Zhang
作者单位	1.Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 201210, Shanghai, China 2.iHuman Institute, ShanghaiTech University, 201210, Shanghai, China 3.School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China 4.Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA 5.State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China 6.State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
第一作者单位	免疫化学研究所; 生命科学与技术学院
通讯作者单位	iHuman研究所; 免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Yiru Wang,Lixia Zhao,Yi Zhang,et al. Cryo-EM structure of Nipah virus RNA polymerase complex[J]. SCIENCE ADVANCES,2024,10(50).
APA	Yiru Wang.,Lixia Zhao.,Yi Zhang.,Yuhan Wang.,Jiao Tang.,...&Heqiao Zhang.(2024).Cryo-EM structure of Nipah virus RNA polymerase complex.SCIENCE ADVANCES,10(50).
MLA	Yiru Wang,et al."Cryo-EM structure of Nipah virus RNA polymerase complex".SCIENCE ADVANCES 10.50(2024).