ShanghaiTech University Knowledge Management System
Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution | |
2017-06-19 | |
发表期刊 | SCIENTIFIC REPORTS
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ISSN | 2045-2322 |
卷号 | 7 |
发表状态 | 已发表 |
DOI | 10.1038/s41598-017-03922-3 |
摘要 | Hydrogen-deuterium exchange (HDX) coupled with mass spectrometry (HDXMS) is a rapid and effective method for localizing and determining protein stability and dynamics. Localization is routinely limited to a peptide resolution of 5 to 20 amino acid residues. HDXMS data can contain information beyond that needed for defining protein stability at single amide resolution. Here we present a method for extracting this information from an HDX dataset to generate a HDXMS protein stability fingerprint. High resolution (HR)-HDXMS was applied to the analysis of a model protein of a spectrin tandem repeat that exemplified an intuitive stability profile based on the linkage of two triple helical repeats connected by a helical linker. The fingerprint recapitulated expected stability maximums and minimums with interesting structural features that corroborate proposed mechanisms of spectrin flexibility and elasticity. HR-HDXMS provides the unprecedented ability to accurately assess protein stability at the resolution of a single amino acid. The determination of HDX stability fingerprints may be broadly applicable in many applications for understanding protein structure and function as well as protein ligand interactions. |
收录类别 | SCI |
语种 | 英语 |
资助项目 | University of California[S97-90] ; University of California[S99-44] ; University of California[L98-30] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000403650300032 |
出版者 | NATURE PUBLISHING GROUP |
WOS关键词 | ELECTRON-TRANSFER DISSOCIATION ; HYDROGEN/DEUTERIUM-EXCHANGE ; RESIDUE RESOLUTION ; PROTEIN DYNAMICS ; SPECTRIN ; MS ; STABILITY ; MECHANISM ; PEPTIDES ; REPEATS |
原始文献类型 | Article |
通讯作者 | Pantazatos, Dionysios P. |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2911 |
专题 | iHuman研究所_特聘教授组_Kurt Wuthrich组 iHuman研究所_PI研究组_徐菲组 |
通讯作者 | Pantazatos, Dionysios P. |
作者单位 | 1.Indiana Univ, Dept Informat & Comp, Bloomington, IN USA 2.Weill Cornell Med, Div Infect Dis, Transplantat Oncol Infect Dis Program, 1300 York Ave, New York, NY 10065 USA 3.GlaxoSmithKline, Platform Technol & Sci, Collegeville Rd, Collegeville, PA 19426 USA 4.ShanghaiTech Univ, iHuman Inst, 99 Haike Rd, Shanghai, Peoples R China 5.Philipps Univ Marburg, Fac Chem, Hans Meerwein Str, D-35043 Marburg, Germany 6.Philipps Univ Marburg, LOEWE Ctr Synthet Microbiol, Hans Meerwein Str, D-35043 Marburg, Germany |
推荐引用方式 GB/T 7714 | Gessner, Chris,Steinchen, Wieland,Bedard, Sabrina,et al. Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution[J]. SCIENTIFIC REPORTS,2017,7. |
APA | Gessner, Chris.,Steinchen, Wieland.,Bedard, Sabrina.,Skinner, John J..,Woods, Virgil L..,...&Pantazatos, Dionysios P..(2017).Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution.SCIENTIFIC REPORTS,7. |
MLA | Gessner, Chris,et al."Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution".SCIENTIFIC REPORTS 7(2017). |
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