Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

doi:10.1038/s41467-018-06627-x

	Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole
	Liu, Dazhi 1; Wei, Yifeng 2; Liu, Xuyang3,4 ; Zhou, Yan 1; Jiang, Li 1; Yin, Jinyu 1; Wang, Feifei 1; Hu, Yiling 1; Urs, Ankanahalli N. Nanjaraj 1; Liu, Yanhong 5; Ang, Ee Lui 2; Zhao, Suwen3,4 ; Zhao, Huimin 2,6; Zhang, Yan 1
	2018-10-11
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	9
发表状态	已发表
DOI	10.1038/s41467-018-06627-x
摘要	Skatole is a malodorous compound that contributes to the characteristic smell of animal faeces. Although skatole has long been known to originate from bacterial tryptophan fermentation, the enzyme catalysing its formation has so far remained elusive. Here we report the use of comparative genomics for the discovery of indoleacetate decarboxylase, an O-2-sensitive glycyl radical enzyme catalysing the decarboxylation of indoleacetate to form skatole as the terminal step of tryptophan fermentation in certain anaerobic bacteria. We describe its biochemical characterization and compare it to other glycyl radical decarboxylases. Indoleacetate decarboxylase may serve as a genetic marker for the identification of skatole-producing environmental and human-associated bacteria, with impacts on human health and the livestock industry.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Science Foundation of China[31570060]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000447075700012
出版者	NATURE PUBLISHING GROUP
WOS关键词	III RIBONUCLEOTIDE-REDUCTASE ; AROMATIC-AMINO-ACIDS ; P-CRESOL ; 4-HYDROXYPHENYLACETATE DECARBOXYLASE ; ACTIVATING ENZYME ; SP NOV. ; 3-METHYLINDOLE ; PROTEIN ; MECHANISMS ; PNEUMOTOXICITY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28116
专题	生命科学与技术学院_博士生 iHuman研究所_PI研究组_赵素文组
通讯作者	Zhao, Suwen; Zhao, Huimin; Zhang, Yan
作者单位	1.Tianjin Univ, Sch Pharmaceut Sci & Technol, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China 2.ASTAR, Inst Chem & Engn Sci, Metab Engn Res Lab, Singapore 138669, Singapore 3.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201202, Peoples R China 5.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China 6.Univ Illinois, Dept Chem & Biomol Engn, 600 South Mathews Ave, Urbana, IL 61801 USA
通讯作者单位	iHuman研究所; 生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Dazhi,Wei, Yifeng,Liu, Xuyang,et al. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole[J]. NATURE COMMUNICATIONS,2018,9.
APA	Liu, Dazhi.,Wei, Yifeng.,Liu, Xuyang.,Zhou, Yan.,Jiang, Li.,...&Zhang, Yan.(2018).Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole.NATURE COMMUNICATIONS,9.
MLA	Liu, Dazhi,et al."Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole".NATURE COMMUNICATIONS 9(2018).