Probing the structures of G protein-coupled receptors with mass spectrometry-based techniques

doi:10.1016/j.ijms.2022.116968

	Probing the structures of G protein-coupled receptors with mass spectrometry-based techniques
	Pan, Chen1 ; Li, Jingwen 2; Yuan, Shijia1,3 ; Shui, Wenqing1,2
	2023
发表期刊	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY (IF:1.6[JCR-2023],1.6[5-Year])
ISSN	1387-3806
卷号	483
发表状态	已发表
DOI	10.1016/j.ijms.2022.116968
摘要	G protein-coupled receptors (GPCRs) are key molecules to regulate numerous physiological processes in the human body, and represent one of the largest protein families as targets for drug development for various human diseases. Understanding the structural basis for GPCR activation, signaling and regulatory mechanisms lays the foundation for structure-based drug design. Compared to X-ray crystallography and cryo-electron microscopy which mainly present static snapshots of protein structures, mass spectrometry (MS)-based structural analysis is able to yield complementary information on the conformational ensemble and dynamics of protein machineries and their interactions with ligands or other proteins. Analysis of GPCR structures with multiple MS-based techniques have facilitated the characterization of structural architecture and conformational dynamics of GPCR proteins complexed with specific ligands and/or downstream signaling partners. In this review, we introduce four MS techniques (chemical cross-linking MS, hydrogen/deuterium exchange MS, native MS and fast photochemical oxidation of proteins MS) and illustrate how they are implemented to probe the structures of GPCR proteins and signaling complexes.
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收录类别	SCOPUS
语种	英语
Scopus 记录号	2-s2.0-85141291625
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251406
专题	iHuman研究所_PI研究组_水雯箐组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Shui, Wenqing
作者单位	1.iHuman Institute,School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China 2.National Facility for Protein Science in Shanghai,Shanghai Advanced Research Institute,Chinese Academy of Science,Shanghai,201210,China 3.University of Chinese Academy of Sciences,Beijing,100049,China
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Pan, Chen,Li, Jingwen,Yuan, Shijia,et al. Probing the structures of G protein-coupled receptors with mass spectrometry-based techniques[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2023,483.
APA	Pan, Chen,Li, Jingwen,Yuan, Shijia,&Shui, Wenqing.(2023).Probing the structures of G protein-coupled receptors with mass spectrometry-based techniques.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,483.
MLA	Pan, Chen,et al."Probing the structures of G protein-coupled receptors with mass spectrometry-based techniques".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 483(2023).