Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue

doi:10.1038/s41467-022-29273-w

	Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue
	Wang, Haofeng1,2,3,4 ; Xiao, Yunjie 1,2,3,4,5; Chen, Xia 1; Zhang, Mengwen 6,7; Sun, Guangxin 6; Wang, Feng 1; Wang, Lin2,3,4 ; Zhang, Hanxiao 1; Zhang, Xiaoyu 1,8,9; Yang, Xin 5; Li, Wenling 1; Wei, Yi 1; Yao, Deqiang 10; Zhang, Bing2,3 ; Li, Jun2,3 ; Cui, Wen 1,5; Wang, Fenghua 1; Chen, Cheng 1; Shen, Wei2,3,4 ; Su, Dan 11,12,13; Bai, Fang2,3,4 ; Huang, Jinhai 1; Ye, Sheng 1; Zhang, Lei 1; Ji, Xiaoyun 14; Wang, Wei 5; Wang, Zefang 1,9; Hochstrasser, Mark 6; Yang, Haitao1,2,3,4,9
	2022-03-25
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
EISSN	2041-1723
卷号	13 期号:1 页码:1608
发表状态	已发表
DOI	10.1038/s41467-022-29273-w
摘要	Wolbachia induced cytoplasmic incompatibility (CI) is caused by linked pairs of genes named cifA and cifB. Here, authors show that the residues at interfaces of the CidA-CidB complex is crucial for their binding and contribute to the diversity of CI. Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. Rescue of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.
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收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000773315800012
出版者	NATURE PORTFOLIO
原始文献类型	Article
Scopus 记录号	2-s2.0-85127221820
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243360
专题	免疫化学研究所_PI研究组_白芳组生命科学与技术学院_PI研究组_沈伟组免疫化学研究所_特聘教授组_饶子和组生命科学与技术学院_博士生免疫化学研究所_PI研究组_杨海涛组免疫化学研究所_PI研究组_李俊组
通讯作者	Wang, Wei; Wang, Zefang; Hochstrasser, Mark; Yang, Haitao
作者单位	1.Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China; 2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 4.Shanghai Clin Res & Trial Ctr, Shanghai, Peoples R China; 5.Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; 6.Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA; 7.Yale Univ, Dept Chem, New Haven, CT USA; 8.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin, Peoples R China; 9.Tianjin Int Joint Acad Biotechnol & Med, Tianjin, Peoples R China; 10.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, State Key Lab Oncogenes & Related Genes, Shanghai, Peoples R China; 11.Sichuan Univ, State Key Lab Biotherapy, Chengdu, Peoples R China; 12.Sichuan Univ, Canc Ctr, West China Hosp, Chengdu, Peoples R China; 13.Collaborat Innovat Ctr Biotherapy, Chengdu, Peoples R China; 14.Nanjing Univ, Sch Life Sci, Dept Biotechnol & Pharmaceut Sci, State Key Lab Pharmaceut Biotechnol, Nanjing, Peoples R China
第一作者单位	免疫化学研究所; 生命科学与技术学院
通讯作者单位	免疫化学研究所; 生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Haofeng,Xiao, Yunjie,Chen, Xia,et al. Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue[J]. NATURE COMMUNICATIONS,2022,13(1):1608.
APA	Wang, Haofeng.,Xiao, Yunjie.,Chen, Xia.,Zhang, Mengwen.,Sun, Guangxin.,...&Yang, Haitao.(2022).Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.NATURE COMMUNICATIONS,13(1),1608.
MLA	Wang, Haofeng,et al."Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue".NATURE COMMUNICATIONS 13.1(2022):1608.