Large-scale multiplexed mosaic CRISPR perturbation in the whole organism

doi:10.1016/j.cell.2022.06.039

	Large-scale multiplexed mosaic CRISPR perturbation in the whole organism
	Liu, Bo1 ; Jing, Zhengyu1 ; Zhang, Xiaoming1 ; Chen, Yuxin1,3,4,5 ; Mao, Shaoshuai1,6 ; Kaundal, Ravinder 2,7; Zou, Yan 1,8; Wei, Ge1 ; Zang, Ying1,9 ; Wang, Xinxin1 ; Lin, Wenyang1 ; Di, Minghui1 ; Sun, Yiwen1 ; Chen, Qin1,10 ; Li, Yongqin1,11 ; Xia, Jing1 ; Sun, Jianlong1 ; Lin, Chao-Po1 ; Huang, Xingxu1 ; Chi, Tian1,2
	2022-08-04
发表期刊	CELL (IF:45.5[JCR-2023],49.0[5-Year])
ISSN	1097-4172
EISSN	1097-4172
卷号	185 期号:16 页码:3008-3024.e16
发表状态	已发表
DOI	10.1016/j.cell.2022.06.039
摘要	Here, we report inducible mosaic animal for perturbation (iMAP), a transgenic platform enabling in situ CRISPR targeting of at least 100 genes in parallel throughout the mouse body. iMAP combines Cre-loxP and CRISPR-Cas9 technologies and utilizes a germline-transmitted transgene carrying a large array of individually floxed, tandemly linked gRNA-coding units. Cre-mediated recombination triggers expression of all the gRNAs in the array but only one of them per cell, converting the mice to mosaic organisms suitable for phenotypic characterization and also for high-throughput derivation of conventional single-gene perturbation lines via breeding. Using gRNA representation as a readout, we mapped a miniature Perturb-Atlas cataloging the perturbations of 90 genes across 39 tissues, which yields rich insights into context-dependent gene functions and provides a glimpse of the potential of iMAP in genome decoding.
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收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000853342700002
出版者	CELL PRESS
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/226422
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_池天组生命科学与技术学院_PI研究组_林照博组生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_PI研究组_孙建龙组生命科学与技术学院_硕士生
通讯作者	Chi, Tian
作者单位	1.ShanghaiTech Univ, Gene Editing Ctr, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Yale Univ, Dept Immunobiol, Med Sch, New Haven, CT 06520 USA 3.Fudan Univ, ENT Inst, Shanghai 200031, Peoples R China 4.Fudan Univ, Dept Otorhinolaryngol, Eye & ENT Hosp, State Key Lab Med Neurobiol, Shanghai 200031, Peoples R China 5.Fudan Univ, MOE Frontiers Ctr Brain Sci, NHC Key Lab Hearing Med, Inst Biomed Sci, Shanghai 200031, Peoples R China 6.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China 7.Natl Inst Pharmaceut Educ & Res Raebareli NIPER R, Dept Pharmacol & Toxicol, Lucknow, Uttar Pradesh, India 8.Gaoke Innovat Ctr, Shenzhen Bay Lab, Guangqiao Rd, Shenzhen 518107, Peoples R China 9.Epigen Therapeut Inc, Shanghai 201315, Peoples R China 10.Fudan Univ, Res Ctr Mol Recognit & Synth, Dept Chem, 220 Handan Lu, Shanghai 200433, Peoples R China 11.Qilu Pharmaceut Co Ltd, Shanghai 201210, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Bo,Jing, Zhengyu,Zhang, Xiaoming,et al. Large-scale multiplexed mosaic CRISPR perturbation in the whole organism[J]. CELL,2022,185(16):3008-3024.e16.
APA	Liu, Bo.,Jing, Zhengyu.,Zhang, Xiaoming.,Chen, Yuxin.,Mao, Shaoshuai.,...&Chi, Tian.(2022).Large-scale multiplexed mosaic CRISPR perturbation in the whole organism.CELL,185(16),3008-3024.e16.
MLA	Liu, Bo,et al."Large-scale multiplexed mosaic CRISPR perturbation in the whole organism".CELL 185.16(2022):3008-3024.e16.