CABE-RY: A PAM-ﬂexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

doi:10.1016/j.omtn.2021.07.016

	CABE-RY: A PAM-ﬂexible dual-mutation base editor for reliable modeling of multi-nucleotide variants
	Tao, Wanyu1,2 ; Liu, Qing 3; Huang, Shisheng1,2 ; Wang, Xin1,2 ; Qu, Shiyuan1,2 ; Guo, Junfan1,2 ; Ou, Danfeng 5; Li, Guanglei1,2 ; Zhang, Yu1,2 ; Xu, Xiangmin 3,4; Huang, Xingxu1,2
	2021-12-03
发表期刊	MOLECULAR THERAPY: NUCLEIC ACIDS (IF:6.5[JCR-2023],6.7[5-Year])
ISSN	2162-2531
卷号	26
发表状态	已发表
DOI	10.1016/j.omtn.2021.07.016
摘要	Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical signiﬁcance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent motif (PAM)-ﬂexible SpRY and selected cytosine and adenine base editor-SpRY (CABE-RY), which had the best editing performance, for further study. Characterization and comparison showed that CABE-RY had a smaller DNA editing window and lower RNA off-target edits than the corresponding single base editors. Thus, we have established a versatile tool to efﬁciently simulate MNVs over the genome, which could be very useful for functional studies on MNVs in humans.
关键词	CRISPR MNVs PAM-flexible dual-mutation base editor simultaneous A/C conversions
学科门类	理学
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Science Foundation of China[81830004] ; Leading Talents of Guangdong Province Program[608285568031]
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000730176000010
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/128204
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_硕士生
通讯作者	Xu, Xiangmin; Huang, Xingxu
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Southern Med Univ, Sch Basic Med Sci, Dept Med Genet, Guangzhou 510515, Guangdong, Peoples R China 4.Guangdong Genet Testing Engn Res Ctr, Guangzhou 510515, Guangdong, Peoples R China 5.South China Normal Univ, Int Acad Optoelect Zhaoqing, Zhaoqing 526238, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tao, Wanyu,Liu, Qing,Huang, Shisheng,et al. CABE-RY: A PAM-ﬂexible dual-mutation base editor for reliable modeling of multi-nucleotide variants[J]. MOLECULAR THERAPY: NUCLEIC ACIDS,2021,26.
APA	Tao, Wanyu.,Liu, Qing.,Huang, Shisheng.,Wang, Xin.,Qu, Shiyuan.,...&Huang, Xingxu.(2021).CABE-RY: A PAM-ﬂexible dual-mutation base editor for reliable modeling of multi-nucleotide variants.MOLECULAR THERAPY: NUCLEIC ACIDS,26.
MLA	Tao, Wanyu,et al."CABE-RY: A PAM-ﬂexible dual-mutation base editor for reliable modeling of multi-nucleotide variants".MOLECULAR THERAPY: NUCLEIC ACIDS 26(2021).