CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus

doi:10.1038/s42003-020-0796-5

	CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus
	Wang, Xinjie 1; Ji, Pinpin 2; Fan, Huiying 3; Dang, Lu 4; Wan, Wenwei5 ; Liu, Siyuan5 ; Li, Yanhua 6; Yu, Wenxia5 ; Li, Xiangyang5 ; Ma, Xiaodong 1; Ma, Xu 7; Zhao, Qin 2; Huang, Xingxu5 ; Liao, Ming 3
	2020-02-11
发表期刊	COMMUNICATIONS BIOLOGY (IF:5.2[JCR-2023],5.6[5-Year])
ISSN	2399-3642
EISSN	2399-3642
卷号	3 期号:1
发表状态	已发表
DOI	10.1038/s42003-020-0796-5
摘要	African swine fever virus (ASFV), the aetiological agent of African swine fever (ASF), causes lethal haemorrhagic fever in domestic pigs with high mortality and morbidity and has devastating consequences on the global swine industry. On-site rapid and sensitive detection of ASFV is key to the timely implementation of control. In this study, we developed a rapid, sensitive and instrument-free ASFV detection method based on CRISPR/Cas12a technology and lateral flow detection (named CRISPR/Cas12a-LFD). The limit of detection of CRISPR/Cas12a-LFD is 20 copies of ASFV genomic DNA per reaction, and the detection process can be completed in an hour. The assay showed no cross-reactivity with other swine DNA viruses, and has 100% agreement with real-time PCR detection of ASFV in 149 clinical samples. Overall, the CRISPR/Cas12a-LFD method provides a novel alternative for the portable, simple, sensitive, and specific detection of ASFV and may contribute to the prevention and control of ASF outbreaks.
收录类别	SCI ; SCIE
语种	英语
资助项目	Young Teachers Research and Development Fund from South China Normal University[18KJ06]
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
WOS类目	Biology ; Multidisciplinary Sciences
WOS记录号	WOS:000514621700003
出版者	NATURE PUBLISHING GROUP
WOS关键词	CROSS-PRIMING AMPLIFICATION ; PCR ASSAY ; DIAGNOSIS ; PIG
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/114811
专题	生命科学与技术学院_本科生生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_硕士生物质科学与技术学院_硕士生物质科学与技术学院_本科生生命科学与技术学院_博士生
共同第一作者	Ji, Pinpin; Fan, Huiying; Dang, Lu
通讯作者	Zhao, Qin; Huang, Xingxu; Liao, Ming
作者单位	1.South China Normal Univ, Inst Brain Res & Rehabil, Ctr Studies Psychol Applicat, Guangdong Key Lab Mental Hlth & Cognit Sci, Guangzhou 510631, Peoples R China 2.Northwest A&F Univ, Dept Prevent Vet Med, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China 3.South China Agr Univ, Coll Vet Med, Anim Infect Dis Lab, Guangzhou 510631, Peoples R China 4.Guangzhou Med Univ, Affiliated Canc Hosp & Inst, 78 Hengzhigang Rd, Guangzhou 510095, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Pudong New Area, 100 Haike Rd, Shanghai 201210, Peoples R China 6.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China 7.Natl Res Inst Family Planning, 12 Dahuishi Rd, Beijing 100081, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Xinjie,Ji, Pinpin,Fan, Huiying,et al. CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus[J]. COMMUNICATIONS BIOLOGY,2020,3(1).
APA	Wang, Xinjie.,Ji, Pinpin.,Fan, Huiying.,Dang, Lu.,Wan, Wenwei.,...&Liao, Ming.(2020).CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus.COMMUNICATIONS BIOLOGY,3(1).
MLA	Wang, Xinjie,et al."CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus".COMMUNICATIONS BIOLOGY 3.1(2020).