Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases

doi:10.1126/sciadv.aax8164

	Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases
	Zhang, Tianlong 1; Peli-Gulli, Marie-Pierre 2; Zhang, Zhen1,3 ; Tang, Xin1,3 ; Ye, Jie 1; De Virgilio, Claudio 2; Ding, Jianping1,3
	2019-09
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	5 期号:9
发表状态	已发表
DOI	10.1126/sciadv.aax8164
摘要	The Rag/Gtr GTPases serve as a central module in the nutrient-sensing signaling network upstream of TORC1. In yeast, the anchoring of Gtr1-Gtr2 to membranes depends on the Ego1-Ego2-Ego3 ternary complex (EGO-TC), resulting in an EGO-TC-Gtr1-Gtr2 complex (EGOC). EGO-TC and human Ragulator share no obvious sequence similarities and also differ in their composition with respect to the number of known subunits, which raises the question of how the EGO-TC fulfills its function in recruiting Gtr1-Gtr2. Here, we report the structure of EGOC, in which Ego1 wraps around Ego2, Ego3, and Gtr1-Gtr2. In addition, Ego3 interacts with Gtr1-Gtr2 to stabilize the complex. The functional roles of key residues involved in the assembly are validated by in vivo assays. Our structural and functional data combined demonstrate that EGOC and Ragulator-Rag complex are structurally conserved and that EGO-TC is essential and sufficient to recruit Gtr1-Gtr2 to membranes to ensure appropriate TORC1 signaling.
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收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	Swiss National Science Foundation[310030_166474]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000491128800092
出版者	AMER ASSOC ADVANCEMENT SCIENCE
EI主题词	Signaling
WOS关键词	CRYSTAL-STRUCTURE ; PROTEIN COMPLEX ; CONTROLS TORC1 ; RAGULATOR ; PLASMIDS ; VECTORS ; REVEALS ; GTR1 ; GEF
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/80457
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_丁建平组生命科学与技术学院_博士生
通讯作者	De Virgilio, Claudio; Ding, Jianping
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, State Key Lab Mol Biol,CAS Ctr Excellence Mol Cel, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Fribourg, Dept Biol, CH-1700 Fribourg, Switzerland 3.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Hua Xia Zhong Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Tianlong,Peli-Gulli, Marie-Pierre,Zhang, Zhen,et al. Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases[J]. SCIENCE ADVANCES,2019,5(9).
APA	Zhang, Tianlong.,Peli-Gulli, Marie-Pierre.,Zhang, Zhen.,Tang, Xin.,Ye, Jie.,...&Ding, Jianping.(2019).Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases.SCIENCE ADVANCES,5(9).
MLA	Zhang, Tianlong,et al."Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases".SCIENCE ADVANCES 5.9(2019).