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ShanghaiTech University Knowledge Management System
Sulfuretin protects hepatic cells through regulation of ROS levels and autophagic flux | |
2019-07 | |
发表期刊 | ACTA PHARMACOLOGICA SINICA (IF:6.9[JCR-2023],7.6[5-Year]) |
ISSN | 1671-4083 |
卷号 | 40期号:7页码:908-918 |
发表状态 | 已发表 |
DOI | 10.1038/s41401-018-0193-5 |
摘要 | Palmitate (PA) exposure induces stress conditions featuring ROS accumulation and upregulation of p62 expression, resulting in autophagic flux blockage and cell apoptosis. Sulfuretin (Sul) is a natural product isolated from Rhus verniciflua Stokes; the cytoprotective effect of Sul on human hepatic L02 cells and mouse primary hepatocytes under PA-induced stress conditions was investigated in this study. Sul induced mitophagy by activation of p-TBK1 and LC3 and produced a concomitant decline in p62 expression. Autophagosome formation and mitophagy were assessed by the sensitive dual fluorescence reporter mCherry-EGFP-LC3B, and mitochondrial fragmentation was analyzed using MitoTracker Deep Red FM. A preliminary structure-activity relationship (SAR) for Sul was also investigated, and the phenolic hydroxyl group was found to be pivotal for maintaining the cytoprotective bioactivity of Sul. Furthermore, experiments using flow cytometry and western blots revealed that Sul reversed the cytotoxic effect stimulated by the autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ), and its cytoprotective effect was almost eliminated when the autophagy-related 5 (Atg5) gene was knocked down. These studies suggest that, in addition to its antioxidative effects, Sul stimulates mitophagy and restores impaired autophagic flux, thus protecting hepatic cells from apoptosis, and that Sul has potential future medical applications for hepatoprotection. |
关键词 | sulfuretin palmitate mitophagy autophagic flux |
收录类别 | SCI ; SCIE ; CSCD |
语种 | 英语 |
资助项目 | Shanghai Commission of Science and Technology[16JC1405000] |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
WOS记录号 | WOS:000472610000005 |
出版者 | NATURE PUBLISHING GROUP |
WOS关键词 | OXIDATIVE STRESS ; RHUS-VERNICIFLUA ; HEME OXYGENASE-1 ; FATTY-ACIDS ; MITOCHONDRIAL DYSFUNCTION ; MONITORING AUTOPHAGY ; ACTIVATION ; MECHANISMS ; MITOPHAGY ; APOPTOSIS |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61152 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_特聘教授组_李佳组 |
通讯作者 | Li, Jia; Nan, Fa-jun; Li, Jing-ya |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Materia Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Lu, Yu-ting,Xiao, Yu-feng,Li, Yu-feng,et al. Sulfuretin protects hepatic cells through regulation of ROS levels and autophagic flux[J]. ACTA PHARMACOLOGICA SINICA,2019,40(7):908-918. |
APA | Lu, Yu-ting,Xiao, Yu-feng,Li, Yu-feng,Li, Jia,Nan, Fa-jun,&Li, Jing-ya.(2019).Sulfuretin protects hepatic cells through regulation of ROS levels and autophagic flux.ACTA PHARMACOLOGICA SINICA,40(7),908-918. |
MLA | Lu, Yu-ting,et al."Sulfuretin protects hepatic cells through regulation of ROS levels and autophagic flux".ACTA PHARMACOLOGICA SINICA 40.7(2019):908-918. |
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