PAQR3 Regulates Endoplasmic Reticulum-to-Golgi Trafficking of COPII Vesicle via Interaction with Sec13/Sec31 Coat Proteins

doi:10.1016/j.isci.2018.11.002

	PAQR3 Regulates Endoplasmic Reticulum-to-Golgi Trafficking of COPII Vesicle via Interaction with Sec13/Sec31 Coat Proteins
	Cao, Qianqian 1; Wang, Zheng 1; Wan, Huida 2; Xu, Lijiao1 ; You, Xue1,3 ; Liao, Lujian 2; Chen, Yan1,3
	2018-11-30
发表期刊	ISCIENCE
ISSN	2589-0042
卷号	9 页码:382-+
发表状态	已发表
DOI	10.1016/j.isci.2018.11.002
摘要	Endoplasmic reticulum (ER)-to-Golgi anterograde transport is driven by COPII vesicles mainly composed of a Sec23/Sec24 inner shell and a Sec13/Sec31 outer cage. How COPII vesicles are tethered to the Golgi is not completely understood. We demonstrated here that PAQR3 can facilitate tethering of COPII vesicles to the Golgi. Proximity labeling using PAQR3 fused with APEX2 identified that many proteins involved in intracellular transport are in dose proximity to PAQR3. ER-to-Golgi trafficking of N-acetylgalactosaminyltransferase-2 on removal of brefeldin A is delayed by PAQR3 deletion. RUSH assay also revealed that ER-to-Golgi trafficking is affected by PAQR3. The N-terminal end of PAQR3 can interact with the WD domains of Sec13 and Sec31A. PAQR3 enhances Golgi localization of Sec13 and Sec31A. Furthermore, PAQR3 is localized in the ERGIC and cis-Golgi structures, the acceptor sites for COPII vesicles. Taken together, our study uncovers a role for PAQR3 as a player in regulating ER-to-Golgi transport of COPII vesicles.
收录类别	ESCI ; SCIE ; SCI
资助项目	Chinese Academy of Sciences[XDA12010102] ; Chinese Academy of Sciences[QYZDJ-SSW-SMC008] ; Chinese Academy of Sciences[ZDRW-ZS-2016-8] ; Chinese Academy of Sciences[Y817X11141]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000454331400029
出版者	CELL PRESS
WOS关键词	LIVING CELLS ; TRANSPORT ; PATHWAY ; DEGRADATION ; UBIQUITINATION ; TUMORIGENESIS ; PEROXIDASE ; AUTOPHAGY ; PROVIDES ; SIGNAL
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/48972
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_陈雁组生命科学与技术学院_博士生
通讯作者	Chen, Yan
作者单位	1.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, CAS Key Lab Nutr Metab & Food Safety, Shanghai Inst Biol Sci,Univ Chinese Acad Sci, Shanghai 200031, Peoples R China 2.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Sch Life Sci, Shanghai 200241, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Cao, Qianqian,Wang, Zheng,Wan, Huida,et al. PAQR3 Regulates Endoplasmic Reticulum-to-Golgi Trafficking of COPII Vesicle via Interaction with Sec13/Sec31 Coat Proteins[J]. ISCIENCE,2018,9:382-+.
APA	Cao, Qianqian.,Wang, Zheng.,Wan, Huida.,Xu, Lijiao.,You, Xue.,...&Chen, Yan.(2018).PAQR3 Regulates Endoplasmic Reticulum-to-Golgi Trafficking of COPII Vesicle via Interaction with Sec13/Sec31 Coat Proteins.ISCIENCE,9,382-+.
MLA	Cao, Qianqian,et al."PAQR3 Regulates Endoplasmic Reticulum-to-Golgi Trafficking of COPII Vesicle via Interaction with Sec13/Sec31 Coat Proteins".ISCIENCE 9(2018):382-+.