KDEL Receptor trafficking to the plasma membrane is regu- lated by ACBD3 and Rab4-GTP

doi:10.20944/preprints202212.0254

	KDEL Receptor trafficking to the plasma membrane is regu- lated by ACBD3 and Rab4-GTP
	Tan, Chuanting1,2 ; Du, Yulei1,2 ; Zhu, Lianhui1 ; Jing, Shuaiyang1,2 ; Gao, Jingkai1,2 ; Yue, Xihua1 ; Qian, Yi 1; Lee, Intaek1
	2022-12-14
状态	已发表
摘要	KDEL receptor-1 maintains homeostasis in the early secretory pathway by capturing and retrieving ER-chaperones to the ER during heavy secretory activity. We have previously shown that a Golgi scaffolding protein (ACBD3) facilitates KDEL receptor localization at the Golgi via reg-ulating cargo wave-induced cAMP/PKA-dependent signaling pathway. Unexpectedly, a fraction of the receptor is also known to reside in the plasma membrane as a stress response, although it is largely unknown exactly how KDEL receptor gets exported from the Golgi and travels to the PM. In this study, we sought to investigate the mechanism by which KDEL receptor gets exported from the Golgi en route to the PM and identified two crucial factors that greatly influence post-Golgi traf-ficking of KDEL receptor. We show here that ACBD3 depletion results in significantly increased trafficking of KDEL receptor to the PM via Rab4-positive tubular carriers emanating from the Golgi. Expression of constitutively activated Rab4 mutant (Q72L) increases surface expression of KDEL receptor up to 2~3-fold, whereas expression of GDP-locked Rab4 mutant (S27N) inhibits KDEL re-ceptor localization to the PM. Importantly, KDELR trafficking from the Golgi to the PM is independ-ent of PKA-and Src Kinase-mediated mechanism. Taken together, these results reveal that ACBD3 and Rab4-GTP are key players at the Golgi in regulating KDEL receptor trafficking to the cell sur -face.
关键词	KDEL receptor ACBD3 Rab4 Rab11 Glogi plasma membranes Rab trafficking secretion
语种	英语
DOI	10.20944/preprints202212.0254
相关网址	查看原文
出处	Preprints
收录类别	PPRN.PPRN
WOS记录号	PPRN:33661537
WOS类目	Multidisciplinary Sciences
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/483964
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_James E. Rothman组生命科学与技术学院_硕士生生命科学与技术学院_博士生免疫化学研究所_PI研究组_Katsuhiko Mikoshiba组大科学中心_公共科研平台_大科学装置建设部
通讯作者	Yue, Xihua; Qian, Yi; Lee, Intaek
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tan, Chuanting,Du, Yulei,Zhu, Lianhui,et al. KDEL Receptor trafficking to the plasma membrane is regu- lated by ACBD3 and Rab4-GTP. 2022.