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ShanghaiTech University Knowledge Management System
| Pre-rRNA spatial distribution and functional organization of the nucleolus | |
| 2024-11-03 | |
| 状态 | 已发表 |
| 摘要 | The self-organized nucleolus is where the pre-ribosomal RNA (pre-rRNA) processing and pre-ribosomal ribonucleoprotein (RNP) assembly take place. Here we present the spatiotemporal distribution of pre-rRNA intermediates in the human nucleolus, revealing a segregated spatial distribution of small subunit (SSU) and large subunit (LSU) precursors. Notably, the 5' external transcribed spacer (5' ETS)-containing SSU pre-rRNAs are retained across FC-DFC-PDFC regions, while the internal transcribed spacer 2 (ITS2)-containing LSU pre-rRNAs move to PDFC-GC regions for processing. Inhibiting 5' ETS processing disrupts the SSU pre-rRNA distribution and the nested FC-DFC sub-nucleolar structure. Cells in amniotes possess a multi-layered nucleolus, whereas anamniotes have only a bipartite structure with a merged FC-DFC. Kinetic labeling of pre-rRNA outflow shows a 7-fold higher pace in tetrapartite nucleoli over that in bipartite nucleoli, indicating that the emergence of the nested FC-DFC may facilitate an efficient SSU pre-rRNA processing over the course of evolution. Collectively, depicting the spatiotemporal distribution of pre-rRNAs reveals a key role of processing steps in organizing the multi-layered nucleolus and suggests a possible evolutionary advantage of the multi-layered structure in amniotes. |
| 关键词 | Nucleolus pre-rRNA processing FC/DFC interface bipartite nucleolus multi-layered nucleolus spatial distribution SSU LSU nucleolar substructure |
| 语种 | 英语 |
| DOI | 10.1101/2024.11.02.621631 |
| 相关网址 | 查看原文 |
| 出处 | bioRxiv |
| 收录类别 | PPRN.PPRN |
| WOS记录号 | PPRN:118964437 |
| WOS类目 | Biochemistry & Molecular Biology |
| 资助项目 | National Key R&D Program of China[2021YFA1100203] ; Strategic Priority Research Program of the Chinese Academy of Science[XDB0570000] |
| 文献类型 | 预印本 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/458360 |
| 专题 | 生命科学与技术学院 生命科学与技术学院_特聘教授组_陈玲玲组 生命科学与技术学院_博士生 |
| 通讯作者 | Chen, Ling-Ling |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,Key Lab RNA Sci & Engn, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Fudan Univ, Childrens Hosp, Ctr Mol Med, Shanghai, Peoples R China 4.Fudan Univ, Inst Biomed Sci, Shanghai Key Lab Med Epigenet, Int Lab Med Epigenet & Metab,Minist Sci & Technol, Shanghai, Peoples R China 5.New Cornerstone Sci Lab, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Yu-Hang,Shan, Lin,Yang, Zheng-Hu,et al. Pre-rRNA spatial distribution and functional organization of the nucleolus. 2024. |
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