Regulation of de novo and maintenance DNA methylation by DNA methyltransferases in post-implantation embryos

doi:10.1016/j.jbc.2024.107990

	Regulation of de novo and maintenance DNA methylation by DNA methyltransferases in post-implantation embryos
	Xu, Zhen; Shi, Jiajia; Chen, Qian; Yang, Shuting; Wang, Zilin; Xiao, Biao; Lai, Zhijian; Jing, Yumeng; Li, Yilin; Li, Xiajun
	2024-11-12
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY (IF:4.0[JCR-2023],4.4[5-Year])
ISSN	0021-9258
EISSN	1083-351X
卷号	301 期号:1
发表状态	已发表
DOI	10.1016/j.jbc.2024.107990
摘要	DNA methylation is mainly catalyzed by three DNA methyltransferase (DNMT) proteins in mammals. Usually DNMT1 is considered the primary DNMT for maintenance DNA methylation, whereas DNMT3A and DNMT3B function in de novo DNA methylation. Interestingly, we found DNMT3A and DNMT3B exerted maintenance and de novo DNA methylation in postimplantation mouse embryos. Together with DNMT1, they maintained DNA methylation at some pluripotent genes and lineage marker genes. Germline-derived DNA methylation at the imprinting control regions (ICRs) is stably maintained in embryos. DNMT1 maintained DNA methylation at most ICRs in postimplantation embryos. Surprisingly, DNA methylation was increased at five ICRs after implantation, and two DNMT3 proteins maintained the newly acquired DNA methylation at two of these five ICRs. Intriguingly, DNMT3A and DNMT3B maintained preexisting DNA methylation at four other ICRs, similar to what we found in embryonic stem cells before. These results suggest that DNA methylation is more dynamic than originally thought during embryogenesis including the ICRs of the imprinted regions. DNMT3A and DNMT3B exert both de novo and maintenance DNA methylation functions after implantation. They maintain large portions of newly acquired DNA methylation at variable degrees across the genome in mouse embryos, together with DNMT1. Furthermore, they contribute to maintenance of preexisting DNA methylation at a subset of ICRs as well as in the CpG islands and certain lineage marker gene. These findings may have some implications for the important roles of DNMT proteins in development and human diseases. © 2024 The Authors
关键词	Cell culture Gene encoding Genome Methylation Stem cells Bisulfite-sequencing Control region De novo methylation DNA Methylation DNMT1 DNMT3A DNMT3B Genomic imprinting Implantation Imprinting control region Maintenance methylation Whole-genome bisulphite sequencing
URL	查看原文
收录类别	EI ; SCI
语种	英语
资助项目	Ministry of Science and Technology of the People ' s Republic of China[G2023013054] ; National Natural Science Foundation of China[32370640]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001412473000001
出版者	American Society for Biochemistry and Molecular Biology Inc.
EI入藏号	20245217596099
EI主题词	Mammals
EI分类号	101.1.1 ; 101.3 ; 101.7 ; 101.7.1 ; 103 ; 802.3 Chemical Operations ; 821 Agricultural Equipment and Methods ; Vegetation and Pest Control
原始文献类型	Journal article (JA)
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/452327
专题	生命科学与技术学院生命科学与技术学院_PI研究组_李夏军组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Li, Xiajun
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Xu, Zhen,Shi, Jiajia,Chen, Qian,et al. Regulation of de novo and maintenance DNA methylation by DNA methyltransferases in post-implantation embryos[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2024,301(1).
APA	Xu, Zhen.,Shi, Jiajia.,Chen, Qian.,Yang, Shuting.,Wang, Zilin.,...&Li, Xiajun.(2024).Regulation of de novo and maintenance DNA methylation by DNA methyltransferases in post-implantation embryos.JOURNAL OF BIOLOGICAL CHEMISTRY,301(1).
MLA	Xu, Zhen,et al."Regulation of de novo and maintenance DNA methylation by DNA methyltransferases in post-implantation embryos".JOURNAL OF BIOLOGICAL CHEMISTRY 301.1(2024).