The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase

doi:10.1093/nar/gkz078

	The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase
	Zeng, Qi-Yu 1; Peng, Gui-Xin2 ; Li, Guang2 ; Zhou, Jing-Bo 1; Zheng, Wen-Qiang2 ; Xue, Mei-Qin 1; Wang, En-Duo1,2 ; Zhou, Xiao-Long 1
	2019-04-08
发表期刊	NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN	0305-1048
卷号	47 期号:6 页码:3072-3085
发表状态	已发表
DOI	10.1093/nar/gkz078
摘要	Alanyl-tRNA synthetases (AlaRSs) from three domains of life predominantly rely on a single wobble base pair, G3-U70, of tRNA(Ala) as a major determinant. However, this base pair is divergent in human mitochondrial tRNA(Ala), but instead with a translocated G5-U68. How human mitochondrial AlaRS (hmtAlaRS) recognizes tRNA(Ala), in particular, in the acceptor stem region, remains unknown. In the present study, we found that hmtAlaRS is a monomer and recognizes mitochondrial tRNA(Ala) in a G3-U70-independent manner, requiring several elements in the acceptor stem. In addition, we found that hmtAlaRS misactivates noncognate Gly and catalyzes strong transfer RNA (tRNA)-independent pre-transfer editing for Gly. A completely conserved residue outside of the editing active site, Arg(663), likely functions as a tRNA translocation determinant to facilitate tRNA entry into the editing domain during editing. Finally, we investigated the effects of the severe infantile-onset cardiomyopathy-associated R592W mutation of hmtAlaRS on the canonical enzymatic activities of hmtAlaRS. Overall, our results provide fundamental information about tRNA recognition and deepen our understanding of translational quality control mechanisms by hmtAlaRS.
收录类别	SCI ; SCIE
语种	英语
资助项目	Youth Innovation Promotion Association (Chinese Academy of Sciences)[Y119S41291]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000467964800032
出版者	OXFORD UNIV PRESS
WOS关键词	AMINOACYL-TRANSFER-RNA ; MAJOR DETERMINANT ; ACCEPTOR HELIX ; BASE-PAIR ; IDENTITY ; PROTEIN ; SEQUENCE ; DOMAIN ; CELL
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/40900
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_王恩多组生命科学与技术学院_博士生
通讯作者	Wang, En-Duo; Zhou, Xiao-Long
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol,Ctr Excellence Mol Cell Sc, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zeng, Qi-Yu,Peng, Gui-Xin,Li, Guang,et al. The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase[J]. NUCLEIC ACIDS RESEARCH,2019,47(6):3072-3085.
APA	Zeng, Qi-Yu.,Peng, Gui-Xin.,Li, Guang.,Zhou, Jing-Bo.,Zheng, Wen-Qiang.,...&Zhou, Xiao-Long.(2019).The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase.NUCLEIC ACIDS RESEARCH,47(6),3072-3085.
MLA	Zeng, Qi-Yu,et al."The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase".NUCLEIC ACIDS RESEARCH 47.6(2019):3072-3085.