MiniMounter: A low-cost miniaturized microscopy development toolkit for image quality control and enhancement

doi:10.1002/jbio.202300214

	MiniMounter: A low-cost miniaturized microscopy development toolkit for image quality control and enhancement
	Zhu, Xinyi1 ; Gu, Liangtao1 ; Li, Rui2,3 ; Chen, Liang1 ; Chen, Jingying1 ; Zhou, Ning2,5 ; Ren, Wuwei1,4
	2023-10
发表期刊	JOURNAL OF BIOPHOTONICS (IF:2.0[JCR-2023],2.6[5-Year])
ISSN	1864-063X
EISSN	1864-0648
卷号	17 期号:2
发表状态	已发表
DOI	10.1002/jbio.202300214
摘要	Head-mounted miniaturized fluorescence microscopy (Miniscope) has emerged as a significant tool in neuroscience, particularly for behavioral studies in awake rodents. However, the challenges of image quality control and standardization persist for both Miniscope users and developers. In this study, we propose a cost-effective and comprehensive toolkit named MiniMounter. This toolkit comprises a hardware platform that offers customized grippers and four-degree-of-freedom adjustment for Miniscope, along with software that integrates displacement control, image quality evaluation, and enhancement of 3D visualization. Our toolkit makes it feasible to accurately characterize Miniscope. Furthermore, MiniMounter enables auto-focusing and 3D imaging for Miniscope prototypes that possess solely a 2D imaging function, as demonstrated in phantom and animal experiments. Overall, the implementation of MiniMounter effectively enhances image quality, reduces the time required for experimental operations and image evaluation, and consequently accelerates the development and research cycle for both users and developers within the Miniscope community.
关键词	brain imaging deconvolution fluorescence imaging image quality control miniaturized microscopy
URL	查看原文
收录类别	SCI ; EI
语种	英语
资助项目	The research leading to these results was funded in part by the National Science Foundation of China (Grant No. 62105205, Wuwei Ren ; Grant No. 32170959, Ning Zhou). This work was also supported by the start-up funding of ShanghaiTech University.[
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Optics
WOS类目	Biochemical Research Methods ; Biophysics ; Optics
WOS记录号	WOS:001098917100001
出版者	WILEY-V C H VERLAG GMBH
EI入藏号	20234615042872
EI主题词	Quality control
EI分类号	461.1 Biomedical Engineering ; 723.2 Data Processing and Image Processing ; 723.5 Computer Applications ; 746 Imaging Techniques ; 911.2 Industrial Economics ; 913.3 Quality Assurance and Control ; 931.1 Mechanics ; 931.4 Quantum Theory ; Quantum Mechanics ; 941.3 Optical Instruments
原始文献类型	Article in Press
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/345963
专题	信息科学与技术学院生命科学与技术学院 iHuman研究所信息科学与技术学院_PI研究组_梁俊睿组生命科学与技术学院_博士生信息科学与技术学院_硕士生信息科学与技术学院_博士生 iHuman研究所_PI研究组_周宁组信息科学与技术学院_PI研究组_任无畏组生物医学工程学院_PI研究组_曹国华组
共同第一作者	Gu, Liangtao
通讯作者	Zhou, Ning; Ren, Wuwei
作者单位	1.ShanghaiTech Univ, Sch Informat Sci & Technol, Shanghai, Peoples R China 2.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.ShanghaiTech Univ, Sch Informat Sci & Technol, Shanghai 201210, Peoples R China 5.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China
第一作者单位	信息科学与技术学院
通讯作者单位	iHuman研究所; 信息科学与技术学院
第一作者的第一单位	信息科学与技术学院
推荐引用方式 GB/T 7714	Zhu, Xinyi,Gu, Liangtao,Li, Rui,et al. MiniMounter: A low-cost miniaturized microscopy development toolkit for image quality control and enhancement[J]. JOURNAL OF BIOPHOTONICS,2023,17(2).
APA	Zhu, Xinyi.,Gu, Liangtao.,Li, Rui.,Chen, Liang.,Chen, Jingying.,...&Ren, Wuwei.(2023).MiniMounter: A low-cost miniaturized microscopy development toolkit for image quality control and enhancement.JOURNAL OF BIOPHOTONICS,17(2).
MLA	Zhu, Xinyi,et al."MiniMounter: A low-cost miniaturized microscopy development toolkit for image quality control and enhancement".JOURNAL OF BIOPHOTONICS 17.2(2023).