Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR

doi:10.1016/j.jsb.2018.03.009

	Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR
	Ma, Cheng-Wei1,2,3 ; Zhang, Jing1,2,3 ; Dong, Xing-Qi1,2,3 ; Lu, Jun-Xia1
	2019-04
发表期刊	JOURNAL OF STRUCTURAL BIOLOGY (IF:3.0[JCR-2023],2.5[5-Year])
ISSN	1047-8477
卷号	206 期号:1 页码:29-35
发表状态	已发表
DOI	10.1016/j.jsb.2018.03.009
摘要	High-order assemblies of amelogenin, the major protein in enamel protein matrix, are believed to act as the template for enamel mineral formation. The Leucine-rich amelogenin (LRAP) is a natural splice-variant of amelogenin, a functional protein in vivo, containing conserved domains of amelogenin. In this work, we showed LRAP aggregates hierarchically into assemblies with various sizes including scattered beads, beads-on-a-string and gel-like precipitations in the presence of both calcium and phosphate ions. Solid-state NMR combined with X-ray diffraction and microscopic techniques, was applied to give a picture of LRAP self-assemblies at the atomic level. Our results, for the first time, confirmed LRAP assemblies with different sizes all contained a consistent rigid segment with beta-sheet secondary structure (residues 12-27) and the beta-sheet segment would further assemble into amyloid-like structures.
关键词	Enamel biomineralization Matrix protein Self-assembly Secondary structure Amyloid structure Solid-state NMR
收录类别	SCI ; SCIE
语种	英语
资助项目	Natural Science Foundation (NSF) of China[31770790]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000465151300004
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	PROTEIN ; NANOSPHERES ; NANORIBBONS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/34332
专题	生命科学与技术学院_硕士生生命科学与技术学院_PI研究组_陆珺霞组生命科学与技术学院_博士生
通讯作者	Lu, Jun-Xia
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Ma, Cheng-Wei,Zhang, Jing,Dong, Xing-Qi,et al. Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR[J]. JOURNAL OF STRUCTURAL BIOLOGY,2019,206(1):29-35.
APA	Ma, Cheng-Wei,Zhang, Jing,Dong, Xing-Qi,&Lu, Jun-Xia.(2019).Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR.JOURNAL OF STRUCTURAL BIOLOGY,206(1),29-35.
MLA	Ma, Cheng-Wei,et al."Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR".JOURNAL OF STRUCTURAL BIOLOGY 206.1(2019):29-35.