An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi

doi:10.1016/j.jbc.2023.104696

	An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi
	Jia, Jie; Tang, Shuocheng; Yue, Xihua; Jing, Shuaiyang; Zhu, Lianhui; Tan, Chuanting; Gao, Jingkai; Du, Yulei; Lee, Intaek; Qian, Yi
	2023-04
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY (IF:4.0[JCR-2023],4.4[5-Year])
ISSN	0021-9258
EISSN	1083-351X
卷号	299 期号:5
发表状态	已发表
DOI	10.1016/j.jbc.2023.104696
摘要	KDEL receptor (KDELR) is a key protein that recycles escaped endoplasmic reticulum (ER) resident proteins from the Golgi apparatus back to the ER and maintains a dynamic balance between these two organelles in the early secretory pathway. Studies have shown that this retrograde transport pathway is partly regulated by two KDELR-interacting proteins, acyl-CoA-binding domain-containing 3 (ACBD3), and cyclic AMP-dependent protein kinase A (PKA). However, whether Golgi-localized ACBD3, which was first discovered as a PKAanchoring protein in mitochondria, directly interacts with PKA at the Golgi and coordinates its signaling in Golgi-to-ER traffic has remained unclear. In this study, we showed that the GOLD domain of ACBD3 directly interacts with the regulatory subunit II (RII) of PKA and effectively recruits PKA holoenzyme to the Golgi. Forward trafficking of proteins from the ER triggers activation of PKA by releasing the catalytic subunit from RII. Furthermore, we determined that depletion of ACBD3 reduces the Golgi fraction of RII, resulting in moderate, but constitutive activation of PKA and KDELR retrograde transport, independent of cargo influx from the ER. Taken together, these data demonstrate that ACBD3 coordinates the protein secretory pathway at the Golgi by facilitating KDELR/PKA-containing protein complex formation.
关键词	Enzymes A-kinase-anchoring proteins Acyl-CoA Acyl-CoA-binding domain-containing 3 Binding domain Endoplasmic reticulum Golgi KDEL receptor Membrane trafficking Protein kinase A Regulatory subunits
URL	查看原文
收录类别	SCI ; EI
语种	英语
资助项目	Shanghai Pujiang Program[16PJ1407]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001009008100001
出版者	ELSEVIER
EI入藏号	20232014088400
EI主题词	Chemical activation
EI分类号	802.2 Chemical Reactions ; 804 Chemical Products Generally
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/294673
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_James E. Rothman组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Lee, Intaek; Qian, Yi
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Jia, Jie,Tang, Shuocheng,Yue, Xihua,et al. An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2023,299(5).
APA	Jia, Jie.,Tang, Shuocheng.,Yue, Xihua.,Jing, Shuaiyang.,Zhu, Lianhui.,...&Qian, Yi.(2023).An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi.JOURNAL OF BIOLOGICAL CHEMISTRY,299(5).
MLA	Jia, Jie,et al."An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi".JOURNAL OF BIOLOGICAL CHEMISTRY 299.5(2023).