| KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP |
| Tan, Chuanting1,2 ; Du, Yulei1,2 ; Zhu, Lianhui1 ; Jing, Shuaiyang1,2 ; Gao, Jingkai1,2 ; Qian, Yi1 ; Yue, Xihua1 ; Lee, Intaek1
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| 2023-04-04
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发表期刊 | CELLS
(IF:5.1[JCR-2023],6.0[5-Year]) |
ISSN | 2073-4409
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EISSN | 2073-4409
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卷号 | 12期号:7页码:1079 |
发表状态 | 已发表
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DOI | 10.3390/cells12071079
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摘要 | KDEL receptor-1 maintains homeostasis in the early secretory pathway by capturing and
retrieving ER chaperones to the ER during heavy secretory activity. Unexpectedly, a fraction of the
receptor is also known to reside in the plasma membrane (PM), although it is largely unknown
exactly how the KDEL receptor gets exported from the Golgi and travels to the PM. We have
previously shown that a Golgi scaffolding protein (ACBD3) facilitates KDEL receptor localization at
the Golgi via the regulating cargo wave-induced cAMP/PKA-dependent signaling pathway. Upon
endocytosis, surface-expressed KDEL receptor undergoes highly complex itineraries through the
Golgi and the endo-lysosomal compartments, where the endocytosed receptor utilizes Rab14A- and
Rab11A-positive recycling endosomes and clathrin-decorated tubulovesicular carriers. In this study,
we sought to investigate the mechanism through which the KDEL receptor gets exported from the
Golgi en route to the PM. We report here that ACBD3 depletion results in greatly increased trafficking
of KDEL receptor to the PM via Rab4A-positive tubular carriers emanating from the Golgi. Expression
of constitutively activated Rab4A mutant (Q72L) increases the surface expression of KDEL receptor
up to 2~3-fold, whereas Rab4A knockdown or the expression of GDP-locked Rab4A mutant (S27N)
inhibits KDEL receptor targeting of the PM. Importantly, KDELR trafficking from the Golgi to the PM
is independent of PKA- and Src kinase-mediated mechanisms. Taken together, these results reveal
that ACBD3 and Rab4A play a key role in regulating KDEL receptor trafficking to the cell surface. |
关键词 | KDEL receptor
ACBD3
Rab4A
Rab11A
Golgi
plasma membrane
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URL | 查看原文
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收录类别 | SCI
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语种 | 英语
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WOS研究方向 | Cell Biology
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WOS类目 | Cell Biology
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WOS记录号 | WOS:000969618500001
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出版者 | MDPI
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文献类型 | 期刊论文
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条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/292204
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专题 | 生命科学与技术学院 生命科学与技术学院_特聘教授组_James E. Rothman组 生命科学与技术学院_硕士生 生命科学与技术学院_博士生
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通讯作者 | Yue, Xihua; Lee, Intaek |
作者单位 | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
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第一作者单位 | 生命科学与技术学院
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通讯作者单位 | 生命科学与技术学院
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第一作者的第一单位 | 生命科学与技术学院
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推荐引用方式 GB/T 7714 |
Tan, Chuanting,Du, Yulei,Zhu, Lianhui,et al. KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP[J].
CELLS,2023,12(7):1079.
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APA |
Tan, Chuanting.,Du, Yulei.,Zhu, Lianhui.,Jing, Shuaiyang.,Gao, Jingkai.,...&Lee, Intaek.(2023).KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP.CELLS,12(7),1079.
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MLA |
Tan, Chuanting,et al."KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP".CELLS 12.7(2023):1079.
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