KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP
2023-04-04
发表期刊CELLS (IF:5.1[JCR-2023],6.0[5-Year])
ISSN2073-4409
EISSN2073-4409
卷号12期号:7页码:1079
发表状态已发表
DOI10.3390/cells12071079
摘要

KDEL receptor-1 maintains homeostasis in the early secretory pathway by capturing and
retrieving ER chaperones to the ER during heavy secretory activity. Unexpectedly, a fraction of the
receptor is also known to reside in the plasma membrane (PM), although it is largely unknown
exactly how the KDEL receptor gets exported from the Golgi and travels to the PM. We have
previously shown that a Golgi scaffolding protein (ACBD3) facilitates KDEL receptor localization at
the Golgi via the regulating cargo wave-induced cAMP/PKA-dependent signaling pathway. Upon
endocytosis, surface-expressed KDEL receptor undergoes highly complex itineraries through the
Golgi and the endo-lysosomal compartments, where the endocytosed receptor utilizes Rab14A- and
Rab11A-positive recycling endosomes and clathrin-decorated tubulovesicular carriers. In this study,
we sought to investigate the mechanism through which the KDEL receptor gets exported from the
Golgi en route to the PM. We report here that ACBD3 depletion results in greatly increased trafficking
of KDEL receptor to the PM via Rab4A-positive tubular carriers emanating from the Golgi. Expression
of constitutively activated Rab4A mutant (Q72L) increases the surface expression of KDEL receptor
up to 2~3-fold, whereas Rab4A knockdown or the expression of GDP-locked Rab4A mutant (S27N)
inhibits KDEL receptor targeting of the PM. Importantly, KDELR trafficking from the Golgi to the PM
is independent of PKA- and Src kinase-mediated mechanisms. Taken together, these results reveal
that ACBD3 and Rab4A play a key role in regulating KDEL receptor trafficking to the cell surface.

关键词KDEL receptor ACBD3 Rab4A Rab11A Golgi plasma membrane
URL查看原文
收录类别SCI
语种英语
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000969618500001
出版者MDPI
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/292204
专题生命科学与技术学院
生命科学与技术学院_特聘教授组_James E. Rothman组
生命科学与技术学院_硕士生
生命科学与技术学院_博士生
通讯作者Yue, Xihua; Lee, Intaek
作者单位
1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
第一作者单位生命科学与技术学院
通讯作者单位生命科学与技术学院
第一作者的第一单位生命科学与技术学院
推荐引用方式
GB/T 7714
Tan, Chuanting,Du, Yulei,Zhu, Lianhui,et al. KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP[J]. CELLS,2023,12(7):1079.
APA Tan, Chuanting.,Du, Yulei.,Zhu, Lianhui.,Jing, Shuaiyang.,Gao, Jingkai.,...&Lee, Intaek.(2023).KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP.CELLS,12(7),1079.
MLA Tan, Chuanting,et al."KDEL Receptor Trafficking to the Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP".CELLS 12.7(2023):1079.
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