alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons

doi:10.1093/cercor/bhx225

	alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons
	Wei, Dan 1; Gao, Nannan 1; Li, Lei 1; Zhu, Jing-Xiang 1; Diao, Lei 1; Huang, Jiansong1,4 ; Han, Qing-Jian 1; Wang, Shaogang1,4 ; Xue, Huaqing 1; Wang, Qiong 1; Wu, Qing-Feng 2,3; Zhang, Xu2,3,4 ; Bao, Lan1,4
	2018-09
发表期刊	CEREBRAL CORTEX (IF:2.9[JCR-2023],3.7[5-Year])
ISSN	1047-3211
卷号	28 期号:9 页码:3332-3346
发表状态	已发表
DOI	10.1093/cercor/bhx225
摘要	Axon growth is tightly controlled to establish functional neural circuits during brain development. Despite the belief that cytoskeletal dynamics is critical for cell morphology, how microtubule acetylation regulates axon development in the mammalian central nervous system remains unclear. Here, we report that loss of a-tubulin acetylation by ablation of MEC-17 in mice predisposes neurons to axon overbranching and overgrowth. Introduction of MEC-17(F183A) lacking alpha-tubulin acetyltransferase activity into MEC-17-deficient neurons failed to rescue axon defects. Moreover, loss of alpha-tubulin acetylation led to increases in microtubule debundling, microtubule invasion into filopodia and growth cones, and microtubule plus-end dynamics along the axon. Taxol application dampened microtubule hyperdynamics and suppressed axon overbranching and overgrowth in MEC-17-deficient neurons. Thus, our study reveals that alpha-tubulin acetylation acts as a brake for axon overbranching and overgrowth by dampening microtubule dynamics, providing insight into the role of microtubule post-translational modifications in regulating neural development.
关键词	axon branching MEC-17 microtubule dynamics alpha-tubulin acetylation
收录类别	SCI ; SCIE
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19000000]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000443545600021
出版者	OXFORD UNIV PRESS INC
WOS关键词	MOLECULAR-BASIS ; ACETYLTRANSFERASE ; MEC-17 ; MECHANISMS ; INSTABILITY ; DEFICIENCY ; ELONGATION ; RESISTANCE ; MIGRATION ; TRANSPORT
原始文献类型	Article
引用统计	被引频次：44[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27695
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_鲍岚组生命科学与技术学院_特聘教授组_张旭组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Bao, Lan
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biol Sci,Univ Chinese Acad Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, State Key Lab Neurosci, CAS Ctr Excellence Brain Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wei, Dan,Gao, Nannan,Li, Lei,et al. alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons[J]. CEREBRAL CORTEX,2018,28(9):3332-3346.
APA	Wei, Dan.,Gao, Nannan.,Li, Lei.,Zhu, Jing-Xiang.,Diao, Lei.,...&Bao, Lan.(2018).alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons.CEREBRAL CORTEX,28(9),3332-3346.
MLA	Wei, Dan,et al."alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons".CEREBRAL CORTEX 28.9(2018):3332-3346.