上海科技大学知识管理系统

Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.

本发明提供了融合蛋白，其包含载脂蛋白B mRNA编辑酶催化亚基3A(apOBEC3A)和成簇的规则间隔的短回文重复(CRISPR)相关(CAS)蛋白，任选地还包含尿嘧啶糖基化酶抑制剂(UGI)。 这样的融合蛋白能够通过将胞嘧啶脱氨为尿嘧啶在DNA中进行碱基编辑，即使当胞嘧啶处于GPC上下文或被甲基化时。