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Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB | |
2022 | |
发表期刊 | PROTEIN & CELL (IF:13.6[JCR-2023],17.7[5-Year]) |
ISSN | 1674-800X |
EISSN | 1674-8018 |
发表状态 | 已发表 |
DOI | 暂无 |
摘要 | The adenosine 5MODIFIER LETTER PRIME-triphosphate (ATP)-binding cassette (ABC) transporter, IrtAB, plays a vital role in the replication and viability of Mycobacterium tuberculosis (Mtb), where its function is to import iron-loaded siderophores. Unusually, it adopts the canonical type IV exporter fold. Herein, we report the structure of unliganded Mtb IrtAB and its structure in complex with ATP, ADP, or ATP analogue (AMP-PNP) at resolutions ranging from 2.8 to 3.5 angstrom. The structure of IrtAB bound ATP-Mg2+ shows a "head-to-tail" dimer of nucleotide-binding domains (NBDs), a closed amphipathic cavity within the transmembrane domains (TMDs), and a metal ion liganded to three histidine residues of IrtA in the cavity. Cryo-electron microscopy (Cryo-EM) structures and ATP hydrolysis assays show that the NBD of IrtA has a higher affinity for nucleotides and increased ATPase activity compared with IrtB. Moreover, the metal ion located in the TM region of IrtA is critical for the stabilization of the conformation of IrtAB during the transport cycle. This study provides a structural basis to explain the ATP-driven conformational changes that occur in IrtAB. |
关键词 | ABC exporter-like importer iron-loaded siderophore IrtAB Mycobacterium tuberculosis ABC transporter |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2022YFC2302900] ; National Natural Science Foundation of China[32171217] ; Shanghai Sailing Program[21YF1429700] ; Young Elite Scientists Sponsorship Program by CAST[2021QNRC001] ; Shanghai Municipal Science and Technology Major Project[20XD1422900] ; Science and Technology Commission of Shanghai Municipality[LG202101-01-08] ; null[ZD2021CY001] |
WOS研究方向 | Cell Biology |
WOS类目 | Cell Biology |
WOS记录号 | WOS:000945210500001 |
出版者 | OXFORD UNIV PRESS |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/246898 |
专题 | 免疫化学研究所 免疫化学研究所_特聘教授组_饶子和组 生命科学与技术学院_博士生 免疫化学研究所_PI研究组_杨海涛组 免疫化学研究所_PI研究组_白芳组 |
通讯作者 | Yang, Haitao; Rao, Zihe; Zhang, Bing |
作者单位 | 1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100101, Peoples R China 4.Shanghai Clin Res & Trial Ctr, Shanghai 201210, Peoples R China 5.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300353, Peoples R China 6.Tsinghua Univ, Lab Struct Biol, Beijing 100084, Peoples R China 7.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia 8.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
第一作者单位 | 免疫化学研究所 |
通讯作者单位 | 免疫化学研究所 |
第一作者的第一单位 | 免疫化学研究所 |
推荐引用方式 GB/T 7714 | Sun, Shan,Gao, Yan,Yang, Xiaolin,et al. Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB[J]. PROTEIN & CELL,2022. |
APA | Sun, Shan.,Gao, Yan.,Yang, Xiaolin.,Yang, Xiuna.,Hu, Tianyu.,...&Zhang, Bing.(2022).Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB.PROTEIN & CELL. |
MLA | Sun, Shan,et al."Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB".PROTEIN & CELL (2022). |
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