Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB
2022
发表期刊PROTEIN & CELL (IF:13.6[JCR-2023],17.7[5-Year])
ISSN1674-800X
EISSN1674-8018
发表状态已发表
DOI暂无
摘要

The adenosine 5MODIFIER LETTER PRIME-triphosphate (ATP)-binding cassette (ABC) transporter, IrtAB, plays a vital role in the replication and viability of Mycobacterium tuberculosis (Mtb), where its function is to import iron-loaded siderophores. Unusually, it adopts the canonical type IV exporter fold. Herein, we report the structure of unliganded Mtb IrtAB and its structure in complex with ATP, ADP, or ATP analogue (AMP-PNP) at resolutions ranging from 2.8 to 3.5 angstrom. The structure of IrtAB bound ATP-Mg2+ shows a "head-to-tail" dimer of nucleotide-binding domains (NBDs), a closed amphipathic cavity within the transmembrane domains (TMDs), and a metal ion liganded to three histidine residues of IrtA in the cavity. Cryo-electron microscopy (Cryo-EM) structures and ATP hydrolysis assays show that the NBD of IrtA has a higher affinity for nucleotides and increased ATPase activity compared with IrtB. Moreover, the metal ion located in the TM region of IrtA is critical for the stabilization of the conformation of IrtAB during the transport cycle. This study provides a structural basis to explain the ATP-driven conformational changes that occur in IrtAB.

关键词ABC exporter-like importer iron-loaded siderophore IrtAB Mycobacterium tuberculosis ABC transporter
URL查看原文
收录类别SCI
语种英语
资助项目National Key Research and Development Program of China[2022YFC2302900] ; National Natural Science Foundation of China[32171217] ; Shanghai Sailing Program[21YF1429700] ; Young Elite Scientists Sponsorship Program by CAST[2021QNRC001] ; Shanghai Municipal Science and Technology Major Project[20XD1422900] ; Science and Technology Commission of Shanghai Municipality[LG202101-01-08] ; null[ZD2021CY001]
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000945210500001
出版者OXFORD UNIV PRESS
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/246898
专题免疫化学研究所
免疫化学研究所_特聘教授组_饶子和组
生命科学与技术学院_博士生
免疫化学研究所_PI研究组_杨海涛组
免疫化学研究所_PI研究组_白芳组
通讯作者Yang, Haitao; Rao, Zihe; Zhang, Bing
作者单位
1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100101, Peoples R China
4.Shanghai Clin Res & Trial Ctr, Shanghai 201210, Peoples R China
5.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300353, Peoples R China
6.Tsinghua Univ, Lab Struct Biol, Beijing 100084, Peoples R China
7.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia
8.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China
第一作者单位免疫化学研究所
通讯作者单位免疫化学研究所
第一作者的第一单位免疫化学研究所
推荐引用方式
GB/T 7714
Sun, Shan,Gao, Yan,Yang, Xiaolin,et al. Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB[J]. PROTEIN & CELL,2022.
APA Sun, Shan.,Gao, Yan.,Yang, Xiaolin.,Yang, Xiuna.,Hu, Tianyu.,...&Zhang, Bing.(2022).Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB.PROTEIN & CELL.
MLA Sun, Shan,et al."Cryo-EM structures for the Mycobacterium tuberculosis iron-loaded siderophore transporter IrtAB".PROTEIN & CELL (2022).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Sun, Shan]的文章
[Gao, Yan]的文章
[Yang, Xiaolin]的文章
百度学术
百度学术中相似的文章
[Sun, Shan]的文章
[Gao, Yan]的文章
[Yang, Xiaolin]的文章
必应学术
必应学术中相似的文章
[Sun, Shan]的文章
[Gao, Yan]的文章
[Yang, Xiaolin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。