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ShanghaiTech University Knowledge Management System
Cryo-EM study of patched in lipid nanodisc suggests a structural basis for its clustering in caveolae | |
2021-11-04 | |
发表期刊 | STRUCTURE (IF:4.4[JCR-2023],4.3[5-Year]) |
ISSN | 0969-2126 |
EISSN | 1878-4186 |
卷号 | 29期号:11页码:1286-+ |
发表状态 | 正式接收 |
DOI | 10.1016/j.str.2021.06.004 |
摘要 | The 12-transmembrane protein Patched (Ptc1) acts as a suppressor for Hedgehog (Hh) signaling by depleting sterols in the cytoplasmic membrane leaflet that are required for the activation of downstream regulators. The positive modulator Hh inhibits Ptc1's transporter function by binding to Ptc1 and its co-receptors, which are locally concentrated in invaginated microdomains known as caveolae. Here, we reconstitute the mouse Ptc1 into lipid nanodiscs and determine its structure using single-particle cryoelectron microscopy. The structure is overall similar to those in amphipol and detergents but displays various conformational differences in the transmembrane region. Although most particles show monomers, we observe Ptc1 dimers with distinct interaction patterns and different membrane curvatures, some of which are reminiscent of caveolae. We find that an extramembranous hand-shake region rich in hydrophobic and aromatic residues mediates inter-Ptc1 interactions under different membrane curvatures. Our data provide a plausible framework for Ptc1 clustering in the highly curved caveolae. |
收录类别 | SCIE |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
WOS记录号 | WOS:000718037100009 |
出版者 | CELL PRESS |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243251 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_特聘教授组_赵允组 生命科学与技术学院_硕士生 |
通讯作者 | Qu, Qianhui; Cong, Yao; Zhao, Yun; Li, Dianfan |
作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,State Key Lab Mol, Shanghai Inst Biochem & Cell Biol,State Key Lab C, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China; 3.Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Dept Syst Biol Med, Shanghai 200032, Peoples R China; 4.Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Luo, Yitian,Wan, Guoyue,Zhang, Xiang,et al. Cryo-EM study of patched in lipid nanodisc suggests a structural basis for its clustering in caveolae[J]. STRUCTURE,2021,29(11):1286-+. |
APA | Luo, Yitian.,Wan, Guoyue.,Zhang, Xiang.,Zhou, Xuan.,Wang, Qiuwen.,...&Li, Dianfan.(2021).Cryo-EM study of patched in lipid nanodisc suggests a structural basis for its clustering in caveolae.STRUCTURE,29(11),1286-+. |
MLA | Luo, Yitian,et al."Cryo-EM study of patched in lipid nanodisc suggests a structural basis for its clustering in caveolae".STRUCTURE 29.11(2021):1286-+. |
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