Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation
2014-07-01
Source PublicationBIOTECHNOLOGY ADVANCES
ISSN0734-9750
Volume32Issue:4Pages:853-872
Status已发表
DOI10.1016/j.biotechadv.2014.01.006
AbstractBiomotors were once described into two categories: linear motor and rotation motor. Recently, a third type of biomotor with revolution mechanism without rotation has been discovered. By analogy, rotation resembles the Earth rotating on its axis in a complete cycle every 24 h, while revolution resembles the Earth revolving around the Sun one circle per 365 days (see animations http://nanobio.uky.edu/movie.html). The action of revolution that enables a motor free of coiling and torque has solved many puzzles and debates that have occurred throughout the history of viral DNA packaging motor studies. It also settles the discrepancies concerning the structure, stoichiometry, and functioning of DNA translocation motors. This review uses bacteriophages Phi29, HK97, SPP1, P22, T4, and T7 as well as bacterial DNA translocase FtsK and SpollIE or the large eukaryotic dsDNA viruses such as mimivirus and vaccinia virus as examples to elucidate the puzzles. These motors use ATPase, some of which have been confirmed to be a hexamer, to revolve around the dsDNA sequentially. ATP binding induces conformational change and possibly an entropy alteration in ATPase to a high affinity toward dsDNA; but ATP hydrolysis triggers another entropic and conformational change in ATPase to a low affinity for DNA, by which dsDNA is pushed toward an adjacent ATPase subunit. The rotation and revolution mechanisms can be distinguished by the size of channel: the channels of rotation motors are equal to or smaller than 2 nm, that is the size of dsDNA, whereas channels of revolution motors are larger than 3 nm. Rotation motors use parallel threads to operate with a right-handed channel, while revolution motors use a left-handed channel to drive the right-handed DNA in an anti-chiral arrangement. Coordination of several vector factors in the same direction makes viral DNA-packaging motors unusually powerful and effective. Revolution mechanism that avoids DNA coiling in translocating the lengthy genomic dsDNA helix could be advantageous for cell replication such as bacterial binary fission and cell mitosis without the need for topoisomerase or helicase to consume additional energy. (C) 2014 Elsevier Inc. All rights recerved.
KeywordBionanomotor One-way traffic mechanism DNA packaging Virus assembly Bionanotechnology Binary fission Chromosome segregation DNA repair Holliday junction Homologous recombination
Indexed BySCI ; EI
Language英语
Funding ProjectNIH[R01 EB003730] ; NIH[R01 EB012135] ; NIH[U01 CA151648]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000338818300015
PublisherPERGAMON-ELSEVIER SCIENCE LTD
EI Accession Number20142417818760
EI KeywordsBacteriophages ; DNA ; Rotation
EI Classification NumberBiological Materials and Tissue Engineering:461.2 ; Immunology:461.9.1 ; Mechanical Devices:601.1 ; Electric Motors:705.3
WOS KeywordHEAD-TAIL CONNECTOR ; ESCHERICHIA-COLI RECA ; VIRAL PACKAGING MOTOR ; SINGLE-STRANDED-DNA ; CRYSTAL-STRUCTURE ; BACILLUS-SUBTILIS ; IN-VITRO ; ATPASE ACTIVITY ; DIRECTIONAL TRANSLOCATION ; CRYSTALLOGRAPHIC ANALYSIS
Original Document TypeReview
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2391
CollectioniHuman研究所_特聘教授组_Raymond Stevens组
Corresponding AuthorGuo, Peixuan
Affiliation
1.Univ Kentucky, Marky Canc Ctr, Nano Biotechnol Ctr, Lexington, KY 40536 USA
2.Univ Kentucky, Coll Pharm, Lexington, KY 40536 USA
3.Southwest Baptist Univ, Coll Sci & Math, Dept Biol, Bolivar, MO 65613 USA
4.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China
5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Being 100101, Peoples R China
Recommended Citation
GB/T 7714
Guo, Peixuan,Zhao, Zhengyi,Haak, Jeannie,et al. Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation[J]. BIOTECHNOLOGY ADVANCES,2014,32(4):853-872.
APA Guo, Peixuan.,Zhao, Zhengyi.,Haak, Jeannie.,Wang, Shaoying.,Wu, Dong.,...&Tao Weitao.(2014).Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation.BIOTECHNOLOGY ADVANCES,32(4),853-872.
MLA Guo, Peixuan,et al."Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation".BIOTECHNOLOGY ADVANCES 32.4(2014):853-872.
Files in This Item: Download All
File Name/Size DocType Version Access License
Related Services
Usage statistics
Scholar Google
Similar articles in Scholar Google
[Guo, Peixuan]'s Articles
[Zhao, Zhengyi]'s Articles
[Haak, Jeannie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Guo, Peixuan]'s Articles
[Zhao, Zhengyi]'s Articles
[Haak, Jeannie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Guo, Peixuan]'s Articles
[Zhao, Zhengyi]'s Articles
[Haak, Jeannie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.1016@j.biotechadv.2014.01.006.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.