Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation

doi:10.1016/j.biotechadv.2014.01.006

KMS > iHuman研究所 > 特聘教授组 > Raymond Stevens组

	Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation
	Guo, Peixuan 1,2; Zhao, Zhengyi 1,2; Haak, Jeannie 1,2; Wang, Shaoying 1,2; Wu, Dong4 ; Meng, Bing 5; Tao Weitao 3
	2014-07-01
发表期刊	BIOTECHNOLOGY ADVANCES
ISSN	0734-9750
卷号	32 期号:4 页码:853-872
发表状态	已发表
DOI	10.1016/j.biotechadv.2014.01.006
摘要	Biomotors were once described into two categories: linear motor and rotation motor. Recently, a third type of biomotor with revolution mechanism without rotation has been discovered. By analogy, rotation resembles the Earth rotating on its axis in a complete cycle every 24 h, while revolution resembles the Earth revolving around the Sun one circle per 365 days (see animations http://nanobio.uky.edu/movie.html). The action of revolution that enables a motor free of coiling and torque has solved many puzzles and debates that have occurred throughout the history of viral DNA packaging motor studies. It also settles the discrepancies concerning the structure, stoichiometry, and functioning of DNA translocation motors. This review uses bacteriophages Phi29, HK97, SPP1, P22, T4, and T7 as well as bacterial DNA translocase FtsK and SpollIE or the large eukaryotic dsDNA viruses such as mimivirus and vaccinia virus as examples to elucidate the puzzles. These motors use ATPase, some of which have been confirmed to be a hexamer, to revolve around the dsDNA sequentially. ATP binding induces conformational change and possibly an entropy alteration in ATPase to a high affinity toward dsDNA; but ATP hydrolysis triggers another entropic and conformational change in ATPase to a low affinity for DNA, by which dsDNA is pushed toward an adjacent ATPase subunit. The rotation and revolution mechanisms can be distinguished by the size of channel: the channels of rotation motors are equal to or smaller than 2 nm, that is the size of dsDNA, whereas channels of revolution motors are larger than 3 nm. Rotation motors use parallel threads to operate with a right-handed channel, while revolution motors use a left-handed channel to drive the right-handed DNA in an anti-chiral arrangement. Coordination of several vector factors in the same direction makes viral DNA-packaging motors unusually powerful and effective. Revolution mechanism that avoids DNA coiling in translocating the lengthy genomic dsDNA helix could be advantageous for cell replication such as bacterial binary fission and cell mitosis without the need for topoisomerase or helicase to consume additional energy. (C) 2014 Elsevier Inc. All rights recerved.
关键词	Bionanomotor One-way traffic mechanism DNA packaging Virus assembly Bionanotechnology Binary fission Chromosome segregation DNA repair Holliday junction Homologous recombination
收录类别	SCI ; EI
语种	英语
资助项目	NIH[R01 EB003730] ; NIH[R01 EB012135] ; NIH[U01 CA151648]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000338818300015
出版者	PERGAMON-ELSEVIER SCIENCE LTD
EI入藏号	20142417818760
EI主题词	Bacteriophages ; DNA ; Rotation
EI分类号	Biological Materials and Tissue Engineering:461.2 ; Immunology:461.9.1 ; Mechanical Devices:601.1 ; Electric Motors:705.3
WOS关键词	HEAD-TAIL CONNECTOR ; ESCHERICHIA-COLI RECA ; VIRAL PACKAGING MOTOR ; SINGLE-STRANDED-DNA ; CRYSTAL-STRUCTURE ; BACILLUS-SUBTILIS ; IN-VITRO ; ATPASE ACTIVITY ; DIRECTIONAL TRANSLOCATION ; CRYSTALLOGRAPHIC ANALYSIS
原始文献类型	Review
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2391
专题	iHuman研究所_特聘教授组_Raymond Stevens组
通讯作者	Guo, Peixuan
作者单位	1.Univ Kentucky, Marky Canc Ctr, Nano Biotechnol Ctr, Lexington, KY 40536 USA 2.Univ Kentucky, Coll Pharm, Lexington, KY 40536 USA 3.Southwest Baptist Univ, Coll Sci & Math, Dept Biol, Bolivar, MO 65613 USA 4.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Being 100101, Peoples R China
推荐引用方式 GB/T 7714	Guo, Peixuan,Zhao, Zhengyi,Haak, Jeannie,et al. Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation[J]. BIOTECHNOLOGY ADVANCES,2014,32(4):853-872.
APA	Guo, Peixuan.,Zhao, Zhengyi.,Haak, Jeannie.,Wang, Shaoying.,Wu, Dong.,...&Tao Weitao.(2014).Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation.BIOTECHNOLOGY ADVANCES,32(4),853-872.
MLA	Guo, Peixuan,et al."Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation".BIOTECHNOLOGY ADVANCES 32.4(2014):853-872.