Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2

doi:10.1038/s41564-022-01155-3

	Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2
	Sun, Xiaoyu 1; Yi, Chunyan 1; Zhu, Yuanfei 2; Ding, Longfei 3; Xia, Shuai 2; Chen, Xingchen ; Liu, Mu 2; Gu, Chenjian 2; Lu, Xiao 1; Fu, Yadong ; Chen, Shuangfeng5 ; Zhang, Tianlong 6; Zhang, Yaguang 1; Yang, Zhuo 1; Ma, Liyan 1; Gu, Wangpeng 1; Hu, Gaowei 2; Du, Shujuan 2; Yan, Renhong 7; Fu, Weihui ; Yuan, Songhua 3,4; Qiu, Chenli 3,4; Zhao, Chen 3,4; Zhang, Xiaoyan 3,4; He, Yonghui 1; Qu, Aidong 8; Zhou, Xu 8; Li, Xiuling 8; Wong, Gary 9; Deng, Qiang ; Zhou, Qiang ; Lu, Hongzhou ; Ling, Zhiyang 1; Ding, Jianping 1; Lu, Lu 2,3,4; Xu, Jianqing 3,4; Xie, Youhua 2,10; Sun, Bing1,5
	2022-07-01
发表期刊	NATURE MICROBIOLOGY
ISSN	2058-5276
卷号	7 期号:7
发表状态	已发表
DOI	10.1038/s41564-022-01155-3
摘要	["Frequent outbreaks of coronaviruses underscore the need for antivirals and vaccines that can counter a broad range of coronavirus types. We isolated a human antibody named 76E1 from a COVID-19 convalescent patient, and report that it has broad-range neutralizing activity against multiple alpha- and beta-coronaviruses, including the SARS-CoV-2 variants. 76E1 also binds its epitope in peptides from gamma- and delta-coronaviruses. 76E1 cross-protects against SARS-CoV-2 and HCoV-OC43 infection in both prophylactic and therapeutic murine animal models. Structural and functional studies revealed that 76E1 targets a unique epitope within the spike protein that comprises the highly conserved S2' site and the fusion peptide. The epitope that 76E1 binds is partially buried in the structure of the SARS-CoV-2 spike trimer in the prefusion state, but is exposed when the spike protein binds to ACE2. This observation suggests that 76E1 binds to the epitope at an intermediate state of the spike trimer during the transition from the prefusion to the postfusion state, thereby blocking membrane fusion and viral entry. We hope that the identification of this crucial epitope, which can be recognized by 76E1, will guide epitope-based design of next-generation pan-coronavirus vaccines and antivirals.","A broad-range human antibody with neutralizing activity against various coronaviruses, including SARS-CoV-2, targets a unique epitope within the highly conserved S2' site and the fusion peptide of the spike protein that is exposed during interaction with the host cell. The pan-coronavirus antibody protects mice against infection with SARS-CoV-2 and HCoV-OC43 following prophylactic and therapeutic treatment."]
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2018YFA0507402] ; Key International Partnership Program of the Chinese Academy of Sciences[153D31KYSB20180055] ; National Natural Science Foundation of China["81971921","81761128007","32100751","32100123"] ; National Key Research and Development Program of China[2021YFC2300703] ; Shanghai Municipal Science and Technology Major Project[ZD2021CY001] ; National Major Science and Technology Projects of China["2018ZX10301403","2018ZX10301208"] ; Shanghai Science and Technology Commission[21DZ2291300]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000820135800014
出版者	NATURE PORTFOLIO
引用统计	被引频次：73[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/214744
专题	生命科学与技术学院_本科生生命科学与技术学院_特聘教授组_孙兵组生命科学与技术学院_博士生
通讯作者	Ling, Zhiyang; Ding, Jianping; Lu, Lu; Xu, Jianqing; Xie, Youhua; Sun, Bing
作者单位	1.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, Chinese Acad Sci,State Key Lab Cell Biol, Shanghai, Peoples R China 2.Fudan Univ, Shanghai Inst Infect Dis & Biosecur, Key Lab Med Mol Virol MOE NHC CAMS, Dept Med Microbiol & Parasitol,Sch Basic Med Sci, Shanghai, Peoples R China 3.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China 4.Fudan Univ, Shanghai Med Coll, Inst Biomed Sci, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Shanghai Univ, Shanghai Engn Res Ctr Organ Repair, Peoples Hosp Nantong 6, Inst Geriatr,Nantong Hosp,Sch Med, Nantong, Peoples R China 7.Westlake Univ, Key Lab Struct Biol Zhejiang Prov, Ctr Infect Dis Res, Westlake Lab Life Sci & Biomed,Sch Life Sci, Hangzhou, Peoples R China 8.Shanghai Inst Biol Prod Co Ltd, Shanghai, Peoples R China 9.Chinese Acad Sci, Inst Pasteur Shanghai, Shanghai, Peoples R China 10.Fudan Univ, Shanghai Med Coll, Childrens Hosp, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Sun, Xiaoyu,Yi, Chunyan,Zhu, Yuanfei,et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2[J]. NATURE MICROBIOLOGY,2022,7(7).
APA	Sun, Xiaoyu.,Yi, Chunyan.,Zhu, Yuanfei.,Ding, Longfei.,Xia, Shuai.,...&Sun, Bing.(2022).Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2.NATURE MICROBIOLOGY,7(7).
MLA	Sun, Xiaoyu,et al."Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2".NATURE MICROBIOLOGY 7.7(2022).