Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2
2022-07-01
Source PublicationNATURE MICROBIOLOGY
ISSN2058-5276
Volume7Issue:7
Status已发表
DOI10.1038/s41564-022-01155-3
Abstract["Frequent outbreaks of coronaviruses underscore the need for antivirals and vaccines that can counter a broad range of coronavirus types. We isolated a human antibody named 76E1 from a COVID-19 convalescent patient, and report that it has broad-range neutralizing activity against multiple alpha- and beta-coronaviruses, including the SARS-CoV-2 variants. 76E1 also binds its epitope in peptides from gamma- and delta-coronaviruses. 76E1 cross-protects against SARS-CoV-2 and HCoV-OC43 infection in both prophylactic and therapeutic murine animal models. Structural and functional studies revealed that 76E1 targets a unique epitope within the spike protein that comprises the highly conserved S2' site and the fusion peptide. The epitope that 76E1 binds is partially buried in the structure of the SARS-CoV-2 spike trimer in the prefusion state, but is exposed when the spike protein binds to ACE2. This observation suggests that 76E1 binds to the epitope at an intermediate state of the spike trimer during the transition from the prefusion to the postfusion state, thereby blocking membrane fusion and viral entry. We hope that the identification of this crucial epitope, which can be recognized by 76E1, will guide epitope-based design of next-generation pan-coronavirus vaccines and antivirals.","A broad-range human antibody with neutralizing activity against various coronaviruses, including SARS-CoV-2, targets a unique epitope within the highly conserved S2' site and the fusion peptide of the spike protein that is exposed during interaction with the host cell. The pan-coronavirus antibody protects mice against infection with SARS-CoV-2 and HCoV-OC43 following prophylactic and therapeutic treatment."]
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Indexed BySCI ; SCIE
Language英语
Funding ProjectMinistry of Science and Technology of China[2018YFA0507402] ; Key International Partnership Program of the Chinese Academy of Sciences[153D31KYSB20180055] ; National Natural Science Foundation of China["81971921","81761128007","32100751","32100123"] ; National Key Research and Development Program of China[2021YFC2300703] ; Shanghai Municipal Science and Technology Major Project[ZD2021CY001] ; National Major Science and Technology Projects of China["2018ZX10301403","2018ZX10301208"] ; Shanghai Science and Technology Commission[21DZ2291300]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000820135800014
PublisherNATURE PORTFOLIO
Citation statistics
Cited Times:85[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/214744
Collection生命科学与技术学院_本科生
生命科学与技术学院_特聘教授组_孙兵组
生命科学与技术学院_博士生
Corresponding AuthorLing, Zhiyang; Ding, Jianping; Lu, Lu; Xu, Jianqing; Xie, Youhua; Sun, Bing
Affiliation
1.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, Chinese Acad Sci,State Key Lab Cell Biol, Shanghai, Peoples R China
2.Fudan Univ, Shanghai Inst Infect Dis & Biosecur, Key Lab Med Mol Virol MOE NHC CAMS, Dept Med Microbiol & Parasitol,Sch Basic Med Sci, Shanghai, Peoples R China
3.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
4.Fudan Univ, Shanghai Med Coll, Inst Biomed Sci, Shanghai, Peoples R China
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
6.Shanghai Univ, Shanghai Engn Res Ctr Organ Repair, Peoples Hosp Nantong 6, Inst Geriatr,Nantong Hosp,Sch Med, Nantong, Peoples R China
7.Westlake Univ, Key Lab Struct Biol Zhejiang Prov, Ctr Infect Dis Res, Westlake Lab Life Sci & Biomed,Sch Life Sci, Hangzhou, Peoples R China
8.Shanghai Inst Biol Prod Co Ltd, Shanghai, Peoples R China
9.Chinese Acad Sci, Inst Pasteur Shanghai, Shanghai, Peoples R China
10.Fudan Univ, Shanghai Med Coll, Childrens Hosp, Shanghai, Peoples R China
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Sun, Xiaoyu,Yi, Chunyan,Zhu, Yuanfei,et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2[J]. NATURE MICROBIOLOGY,2022,7(7).
APA Sun, Xiaoyu.,Yi, Chunyan.,Zhu, Yuanfei.,Ding, Longfei.,Xia, Shuai.,...&Sun, Bing.(2022).Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2.NATURE MICROBIOLOGY,7(7).
MLA Sun, Xiaoyu,et al."Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2".NATURE MICROBIOLOGY 7.7(2022).
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