Enhancing Carbohydrate Productivity of Chlorella sp AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry

doi:10.1007/s12010-017-2667-1

	Enhancing Carbohydrate Productivity of Chlorella sp AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry
	Yuan, Yizhong1,2,3 ; Liu, Heng3 ; Li, Xuyang 1,4; Qi, Wenqiang 1,3; Cheng, Dujia1,2,3 ; Tang, Tao 1; Zhao, Quanyu1,3 ; Wei, Wei 1,5; Sun, Yuhan1,3
	2018-06
发表期刊	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (IF:3.1[JCR-2023],2.9[5-Year])
ISSN	0273-2289
卷号	185 期号:2 页码:419-433
发表状态	已发表
DOI	10.1007/s12010-017-2667-1
摘要	Accumulated carbohydrate in microalgae is promising feedstock for bioethanol fermentation. Selection of suitable cultivation conditions in semi-continuous cultivation is critical to achieve a high carbohydrate productivity. In the current study, the effects of macro-nutrient (nitrogen, phosphorus, and sulfur) limitations and light intensity were evaluated for the carbohydrate accumulations of Chlorella sp. AE10 under 10% CO2 conditions. It was shown that nitrogen limitation and high light intensity were effective for improving carbohydrate productivity. The average carbohydrate and biomass productivity in semi-continuous cultivation with 1/4 N medium and 1000 mu mol photons m(-2) s(-1) was 0.673 and 0.93 g L-1 day(-1), respectively. Sulfur and phosphorus limitations could improve the carbohydrate content but they could not enhance the carbohydrate productivity. The cell cycle progression and chlorophyll a were investigated using flow cytometry (FCM). The results showed that macro-nutrient limitation and high light intensity indeed influenced cell cycle progression and led to the formation of polyploid cells along with the carbohydrate accumulation in a certain range. FCM was rapid and accurate method to investigate the operation conditions why 1/4 N, 2 days as a cycle, and high light intensity were optimal ones. In addition, the remaining high level of photosynthesis activity was also important for achieving a high carbohydrate productivity. Dynamic tracking of carbohydrate accumulation is helpful for establishment of a semi-continuous cultivation for enhancing carbohydrate productivity in microalgae.
关键词	Carbohydrate accumulation Chlorella Flow cytometry High light intensity Macro-nutrient limitation
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Natural Science Foundation of China[21576278]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000431503400005
出版者	HUMANA PRESS INC
WOS关键词	MICROALGA TETRASELMIS-SUBCORDIFORMIS ; CELL-CYCLE ; CHLAMYDOMONAS-REINHARDTII ; NEOCHLORIS-OLEOABUNDANS ; IMPROVING CARBOHYDRATE ; BIOMASS COMPOSITION ; LIPID-ACCUMULATION ; STARCH PRODUCTION ; COMMITMENT ; FIXATION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20851
专题	生命科学与技术学院物质科学与技术学院_特聘教授组_孙予罕组生命科学与技术学院_特聘教授组_赵权宇组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Zhao, Quanyu
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, 99 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Shanghai Univ, Sch Life Sci, 99 Shangda Rd, Shanghai 200444, Peoples R China 5.Chinese Acad Sci, Inst Urban Environm, Ctr Excellence Urban Atmospher Environm, Xiamen 361021, Peoples R China
第一作者单位	上海科技大学
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Yuan, Yizhong,Liu, Heng,Li, Xuyang,et al. Enhancing Carbohydrate Productivity of Chlorella sp AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2018,185(2):419-433.
APA	Yuan, Yizhong.,Liu, Heng.,Li, Xuyang.,Qi, Wenqiang.,Cheng, Dujia.,...&Sun, Yuhan.(2018).Enhancing Carbohydrate Productivity of Chlorella sp AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,185(2),419-433.
MLA	Yuan, Yizhong,et al."Enhancing Carbohydrate Productivity of Chlorella sp AE10 in Semi-continuous Cultivation and Unraveling the Mechanism by Flow Cytometry".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 185.2(2018):419-433.