Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex
2017-12
发表期刊CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year])
ISSN1001-0602
卷号27期号:12页码:1503-1520
发表状态已发表
DOI10.1038/cr.2017.145
摘要

Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single-and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1. The crystal structure shows that Poz1 employs two different binding surfaces to interact with Tpz1 and Rap1. Unexpectedly, the structure also reveals that Poz1 adopts a dimeric conformation. Mutational analyses suggest that proper interactions between Tpz1, Poz1, and Rap1 in the shelterin core complex are required for telomere length homeostasis and heterochromatin structure maintenance at telomeres. Structural resemblance between Poz1 and the TRFH domains of other shelterin proteins in fission yeast and humans suggests a model for the evolution of shelterin proteins.

关键词telomere shelterin hub TRFH evolution
收录类别SCI
资助项目Japan Society for the Promotion of Science (JSPS) KAKENHI[17H03606]
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000416728800009
出版者INST BIOCHEMISTRY & CELL BIOLOGY
WOS关键词BINDING PROTEIN TAZ1 ; SINGLE-STRANDED-DNA ; SCHIZOSACCHAROMYCES-POMBE ; SHELTERIN COMPONENTS ; MAINTAIN TELOMERES ; RECRUIT TELOMERASE ; DOMAIN PROTEIN ; END-PROTECTION ; HUMAN POT1 ; TRF2
原始文献类型Article
引用统计
正在获取...
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/14284
专题生命科学与技术学院
生命科学与技术学院_特聘教授组_陈勇组
生命科学与技术学院_博士生
通讯作者Chen, Yong; Kanoh, Junko; Lei, Ming
作者单位
1.Chinese Acad Sci, Univ Chinese Acad Sci, Natl Ctr Prot Sci Shanghai,State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 333 Haike Rd, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai, Peoples R China
3.Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China
4.Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan
5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
6.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Xue, Jing,Chen, Hongwen,Wu, Jian,et al. Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex[J]. CELL RESEARCH,2017,27(12):1503-1520.
APA Xue, Jing.,Chen, Hongwen.,Wu, Jian.,Takeuchi, Miho.,Inoue, Haruna.,...&Lei, Ming.(2017).Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex.CELL RESEARCH,27(12),1503-1520.
MLA Xue, Jing,et al."Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex".CELL RESEARCH 27.12(2017):1503-1520.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Xue, Jing]的文章
[Chen, Hongwen]的文章
[Wu, Jian]的文章
百度学术
百度学术中相似的文章
[Xue, Jing]的文章
[Chen, Hongwen]的文章
[Wu, Jian]的文章
必应学术
必应学术中相似的文章
[Xue, Jing]的文章
[Chen, Hongwen]的文章
[Wu, Jian]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 14284.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。