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Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex | |
2017-12 | |
发表期刊 | CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year]) |
ISSN | 1001-0602 |
卷号 | 27期号:12页码:1503-1520 |
发表状态 | 已发表 |
DOI | 10.1038/cr.2017.145 |
摘要 | Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single-and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1. The crystal structure shows that Poz1 employs two different binding surfaces to interact with Tpz1 and Rap1. Unexpectedly, the structure also reveals that Poz1 adopts a dimeric conformation. Mutational analyses suggest that proper interactions between Tpz1, Poz1, and Rap1 in the shelterin core complex are required for telomere length homeostasis and heterochromatin structure maintenance at telomeres. Structural resemblance between Poz1 and the TRFH domains of other shelterin proteins in fission yeast and humans suggests a model for the evolution of shelterin proteins. |
关键词 | telomere shelterin hub TRFH evolution |
收录类别 | SCI |
资助项目 | Japan Society for the Promotion of Science (JSPS) KAKENHI[17H03606] |
WOS研究方向 | Cell Biology |
WOS类目 | Cell Biology |
WOS记录号 | WOS:000416728800009 |
出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
WOS关键词 | BINDING PROTEIN TAZ1 ; SINGLE-STRANDED-DNA ; SCHIZOSACCHAROMYCES-POMBE ; SHELTERIN COMPONENTS ; MAINTAIN TELOMERES ; RECRUIT TELOMERASE ; DOMAIN PROTEIN ; END-PROTECTION ; HUMAN POT1 ; TRF2 |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/14284 |
专题 | 生命科学与技术学院 生命科学与技术学院_特聘教授组_陈勇组 生命科学与技术学院_博士生 |
通讯作者 | Chen, Yong; Kanoh, Junko; Lei, Ming |
作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Natl Ctr Prot Sci Shanghai,State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 333 Haike Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai, Peoples R China 3.Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China 4.Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan 5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Xue, Jing,Chen, Hongwen,Wu, Jian,et al. Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex[J]. CELL RESEARCH,2017,27(12):1503-1520. |
APA | Xue, Jing.,Chen, Hongwen.,Wu, Jian.,Takeuchi, Miho.,Inoue, Haruna.,...&Lei, Ming.(2017).Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex.CELL RESEARCH,27(12),1503-1520. |
MLA | Xue, Jing,et al."Structure of the fission yeast S-pombe telomeric Tpz1-Poz1-Rap1 complex".CELL RESEARCH 27.12(2017):1503-1520. |
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