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Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing | |
2021-08-11 | |
发表期刊 | CELL COMMUNICATION AND SIGNALING |
ISSN | 1478-811X |
EISSN | 1478-811X |
卷号 | 19期号:1 |
发表状态 | 已发表 |
DOI | 10.1186/s12964-021-00716-z |
摘要 | RNA base editing is potential for cellular function research and genetic diseases treating. There are two main RNA base editors, REPAIR and RESCUE, for in vitro use. REPAIR was developed by fusing inactivated Cas13 (dCas13) with the adenine deaminase domain of ADAR2, which efficiently performs adenosine-to-inosine (A-to-I) RNA editing. RESCUE, which performs both cytidine-to-uridine (C-to-U) and A-to-I RNA editing, was developed by fusing inactivated Cas13 (dCas13) with the evolved ADAR2. However, the relatively low editing efficiency of the RESCUE system limits its broad application. Here, we constructed an enhanced RESCUE (eRESCUE) system; this dPspCas13b-RESCUE-NES system was generated by fusing inactivated PspCas13b with the evolved ADAR2. We determined the endogenous mRNA A-to-I and C-to-U editing efficiency mediated by the dPspCas13b-RESCUE-NES system in HEK-293T cells. This new RNA base editor was then used to induce 177Ser/Gly conversion of inhibitor kappa B kinase beta (IKK beta) by changing the genetic code from AGU to GGU. The results showed that the eRESCUE editor mediates more efficient A-to-I and C-to-U RNA editing than the RESCUE RNA editor, as was previously reported. The 177Ser/Gly conversion of IKK beta, accomplished by converting the genetic code from AGU to GGU, resulted in a decrease in the phosphorylation of IKK beta and downregulation of downstream IKK beta-related genes. In summary, we developed a more efficient RNA base editor, eRESCUE, which may provide a useful tool for biomedical research and genetic disease treatment. |
关键词 | RNA base editing eRESCUE Phosphorylation IKK beta |
URL | 查看原文 |
收录类别 | SCIE |
语种 | 英语 |
WOS研究方向 | Cell Biology |
WOS类目 | Cell Biology |
WOS记录号 | WOS:000684265200001 |
出版者 | BMC |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127994 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_黄行许组 |
共同第一作者 | Wang, Yihan |
通讯作者 | Hu, Xiaoxiang |
作者单位 | 1.China Agr Univ, State Key Lab Agrobiotechnol, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China; 2.China Agr Univ, Coll Biol Sci, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China; 3.Natl Res Inst Family Planning, 12 Dahuisi Rd, Beijing 100081, Peoples R China; 4.Peking Union Med Coll, Grad Sch, 9 Dongdan Santiao, Beijing 100730, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 200031, Peoples R China; 6.China Agr Univ, Coll Anim Sci & Technol, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China; 7.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Guo,Wang, Yihan,Li, Xiangyang,et al. Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing[J]. CELL COMMUNICATION AND SIGNALING,2021,19(1). |
APA | Li, Guo.,Wang, Yihan.,Li, Xiangyang.,Wang, Yuzhe.,Huang, Xingxu.,...&Hu, Xiaoxiang.(2021).Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing.CELL COMMUNICATION AND SIGNALING,19(1). |
MLA | Li, Guo,et al."Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing".CELL COMMUNICATION AND SIGNALING 19.1(2021). |
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