lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription

doi:10.1126/science.abf6582

	lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription
	Wu, Man 1; Xu, Guang 1; Han, Chong 1; Luan, Peng-Fei 1; Xing, Yu-Hang 1,5,6; Nan, Fang 2; Yang, Liang-Zhong 1; Huang, Youkui 1; Yang, Zheng-Hu1,3 ; Shan, Lin 1; Yang, Li2,3 ; Liu, Jiaquan 1; Chen, Ling-Ling1,3,4
	2021-07-30
发表期刊	SCIENCE (IF:44.7[JCR-2023],50.3[5-Year])
ISSN	0036-8075
EISSN	1095-9203
卷号	373 期号:6554 页码:547+
DOI	10.1126/science.abf6582
摘要	RNA polymerase I (Pol I) transcription takes place at the border of the fibrillar center (FC) and the dense fibrillar component (DFC) in the nucleolus. Here, we report that individual spherical FC/DFC units are coated by the DEAD-box RNA helicase DDX21 in human cells. The long noncoding RNA (lncRNA) SLERT binds to DDX21 RecA domains to promote DDX21 to adopt a closed conformation at a substoichiometric ratio through a molecular chaperone-like mechanism resulting in the formation of hypomultimerized and loose DDX21 clusters that coat DFCs, which is required for proper FC/DFC liquidity and Pol I processivity. Our results suggest that SLERT is an RNA regulator that controls the biophysical properties of FC/DFCs and thus ribosomal RNA production.
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000681716000041
出版者	AMER ASSOC ADVANCEMENT SCIENCE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127935
专题	生命科学与技术学院_特聘教授组_陈玲玲组生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_博士生
通讯作者	Liu, Jiaquan; Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,CAS Ctr Excelle, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, 320 Yueyang Rd, Shanghai, Peoples R China; 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci,CAS Key Lab Computat Biol, Shanghai, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou, Peoples R China; 5.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA; 6.Harvard Med Sch, Boston, MA 02114 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wu, Man,Xu, Guang,Han, Chong,et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. SCIENCE,2021,373(6554):547+.
APA	Wu, Man.,Xu, Guang.,Han, Chong.,Luan, Peng-Fei.,Xing, Yu-Hang.,...&Chen, Ling-Ling.(2021).lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.SCIENCE,373(6554),547+.
MLA	Wu, Man,et al."lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription".SCIENCE 373.6554(2021):547+.