Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases

doi:10.1093/jimb/kuab024

	Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases
	Ji, Minghua 1,2; Liu, Yunhui1,3 ; Wu, Haiying4 ; Li, Sijie 1; Duan, Haiyan1,3 ; Shi, Jiping1,2,3 ; Sun, Junsong1,2,3
	2021-06
发表期刊	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN	1367-5435
EISSN	1476-5535
卷号	48 期号:5-6
DOI	10.1093/jimb/kuab024
摘要	Catalases are a large group of enzymes that decompose hydrogen peroxide to oxygen and hydrogen, and have been applied widely in numerous areas. Bacillus subtilis ATCC 6051a is a well-known host strain for high level secretion of heterologous peptides. However, the application of 6051a was seriously hampered by insufficient transformation efficiency. In this study, D-xylose inducible comK was integrated into the genome of B. subtilis ATCC 6051a, generating 164S, a mutant owns a transformation efficiency of 1 000-fold higher than its parent strain, thus allowing gene replacement by double crossover recombination using linear dsDNAs. The efficiency of the flanking arms for homologous recombination was then analyzed. We found that 400 bp was the minimal length of homologous fragments required to initiate efficient recombination in the 164S strain. In addition, DNA cassettes encoding two mesophilic catalases (Orf 2-62 and Orf 2-63) from B. licheniformis were integrated onto 164S. The catalytic properties of recombinant Orf 2-62 and Orf 2-63 were analyzed, and were found to be predominantly secreted into the fermentation broth, although they obviously lack any known secretory signal peptide. This work demonstrated that B. subtilis 164S is an excellent cell tool, not only for its superior secretion capacity, but also for its convenience in genetic modification.
关键词	Bacillus subtilis ATCC 6051a Transformation efficiency Comk Catalase
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收录类别	SCI ; SCI ; SCIE
语种	英语
资助项目	Shanghai Science and Technology Commission[18490722400]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000765909400002
出版者	OXFORD UNIV PRESS
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127783
专题	生命科学与技术学院_博士生物质科学与技术学院_PI研究组_钟超组生命科学与技术学院_特聘教授组_孙俊松组生命科学与技术学院_特聘教授组_史吉平组生命科学与技术学院_硕士生
通讯作者	Shi, Jiping; Sun, Junsong
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Ji, Minghua,Liu, Yunhui,Wu, Haiying,et al. Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2021,48(5-6).
APA	Ji, Minghua.,Liu, Yunhui.,Wu, Haiying.,Li, Sijie.,Duan, Haiyan.,...&Sun, Junsong.(2021).Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,48(5-6).
MLA	Ji, Minghua,et al."Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 48.5-6(2021).