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Structural plasticity of mumps virus nucleocapsids with cryo-EM structures
2021-07-02
发表期刊COMMUNICATIONS BIOLOGY (IF:5.2[JCR-2023],5.6[5-Year])
ISSN2399-3642
卷号4期号:1
发表状态已发表
DOI10.1038/s42003-021-02362-0
摘要
Mumps virus (MuV) is a highly contagious human pathogen and frequently causes worldwide outbreaks despite available vaccines. Similar to other mononegaviruses such as Ebola and rabies, MuV uses a single-stranded negative-sense RNA as its genome, which is enwrapped by viral nucleoproteins into the helical nucleocapsid. The nucleocapsid acts as a scaffold for genome condensation and as a template for RNA replication and transcription. Conformational changes in the MuV nucleocapsid are required to switch between different activities, but the underlying mechanism remains elusive due to the absence of high-resolution structures. Here, we report two MuV nucleoprotein-RNA rings with 13 and 14 protomers, one stacked-ring filament and two nucleocapsids with distinct helical pitches, in dense and hyperdense states, at near-atomic resolutions using cryo-electron microscopy. Structural analysis of these in vitro assemblies indicates that the C-terminal tail of MuV nucleoprotein likely regulates the assembly of helical nucleocapsids, and the C-terminal arm may be relevant for the transition between the dense and hyperdense states of helical nucleocapsids. Our results provide the molecular mechanism for structural plasticity among different MuV nucleocapsids and create a possible link between structural plasticity and genome condensation. Shan et al. describes the high-resolution structures of Nucleoprotein in two different oligomeric states and four different higher-order helical structures. They further describe the structural rearrangements required to transition between the different helical assemblies obtained, highlighting the basis for structural plasticity among different MuV nucleocapsids
收录类别SCI ; SCIE
WOS记录号WOS:000672151800004
出版者NATURE RESEARCH
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127592
专题iHuman研究所_PI研究组_沈庆涛组
生命科学与技术学院_PI研究组_白云组
iHuman研究所_公共科研平台_生命科学电镜平台
生命科学与技术学院_硕士生
物质科学与技术学院_硕士生
生命科学与技术学院_博士生
共同第一作者Su X(苏欣); Li TH(李天昊); Zhang N(张娜); Liu YH(刘蕴辉)
通讯作者Shen QT(沈庆涛)
作者单位
1.iHuman Institute and School of Life Science and Technology, ShanghaiTech University, Shanghai, China
2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
3.University of Chinese Academy of Sciences, Beijing, China
4.State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, China
5.College of Marine Life Sciences, Ocean University of China, Qingdao, China
6.School of Life Science and Technology, ShanghaiTech University, Shanghai, China
第一作者单位iHuman研究所
通讯作者单位iHuman研究所
第一作者的第一单位iHuman研究所
推荐引用方式
GB/T 7714
Dan H,Su X,Li TH,et al. Structural plasticity of mumps virus nucleocapsids with cryo-EM structures[J]. COMMUNICATIONS BIOLOGY,2021,4(1).
APA Dan H.,Su X.,Li TH.,Qin YQ.,Zhang N.,...&Shen QT.(2021).Structural plasticity of mumps virus nucleocapsids with cryo-EM structures.COMMUNICATIONS BIOLOGY,4(1).
MLA Dan H,et al."Structural plasticity of mumps virus nucleocapsids with cryo-EM structures".COMMUNICATIONS BIOLOGY 4.1(2021).
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