Characterization of cotton ARF factors and the role of GhARF2b in fiber development

doi:10.1186/s12864-021-07504-6

	Characterization of cotton ARF factors and the role of GhARF2b in fiber development
	Zhang, Xiufang 1,2; Cao, Junfeng 1,2,3,4; Huang, Chaochen1,2,5 ; Zheng, Zishou 1,2,4; Liu, Xia 6; Shangguan, Xiaoxia 1,2; Wang, Lingjian 1,2; Zhang, Yugao 6; Chen, Zhiwen 1,2,7
	2021-03-22
发表期刊	BMC GENOMICS (IF:3.5[JCR-2023],4.1[5-Year])
ISSN	1471-2164
卷号	22 期号:1
发表状态	已发表
DOI	10.1186/s12864-021-07504-6
摘要	BackgroundCotton fiber is a model system for studying plant cell development. At present, the functions of many transcription factors in cotton fiber development have been elucidated, however, the roles of auxin response factor (ARF) genes in cotton fiber development need be further explored.ResultsHere, we identify auxin response factor (ARF) genes in three cotton species: the tetraploid upland cotton G. hirsutum, which has 73 ARF genes, and its putative extent parental diploids G. arboreum and G. raimondii, which have 36 and 35 ARFs, respectively. Ka and Ks analyses revealed that in G. hirsutum ARF genes have undergone asymmetric evolution in the two subgenomes. The cotton ARFs can be classified into four phylogenetic clades and are actively expressed in young tissues. We demonstrate that GhARF2b, a homolog of the Arabidopsis AtARF2, was preferentially expressed in developing ovules and fibers. Overexpression of GhARF2b by a fiber specific promoter inhibited fiber cell elongation but promoted initiation and, conversely, its downregulation by RNAi resulted in fewer but longer fiber. We show that GhARF2b directly interacts with GhHOX3 and represses the transcriptional activity of GhHOX3 on target genes.ConclusionOur results uncover an important role of the ARF factor in modulating cotton fiber development at the early stage.
关键词	Cotton GhARF2b Fiber elongation Fiber initiation
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收录类别	SCIE ; SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000632938700003
出版者	BMC
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126154
专题	生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Chen, Zhiwen
作者单位	1.Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China; 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China; 3.Chinese Acad Sci, Plant Stress Biol Ctr, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China; 4.Univ Chinese Acad Sci, Shanghai 200032, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 6.Esquel Grp, 25 Harbour Rd, Hong Kong, Peoples R China; 7.Shanxi Datong Univ, Inst Carbon Mat Sci, Datong 037009, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiufang,Cao, Junfeng,Huang, Chaochen,et al. Characterization of cotton ARF factors and the role of GhARF2b in fiber development[J]. BMC GENOMICS,2021,22(1).
APA	Zhang, Xiufang.,Cao, Junfeng.,Huang, Chaochen.,Zheng, Zishou.,Liu, Xia.,...&Chen, Zhiwen.(2021).Characterization of cotton ARF factors and the role of GhARF2b in fiber development.BMC GENOMICS,22(1).
MLA	Zhang, Xiufang,et al."Characterization of cotton ARF factors and the role of GhARF2b in fiber development".BMC GENOMICS 22.1(2021).