Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography

doi:10.1126/sciadv.abc8262

	Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography
	White, Kate L.1,2; Singla, Jitin 1,3; Loconte, Valentina4 ; Chen, Jian-Hua 2,5; Ekman, Axel 5; Sun, Liping4 ; Zhang, Xianjun 1; Francis, John Paul 6; Li, Angdi4 ; Lin, Wen 7; Tseng, Kaylee 1; McDermott, Gerry 2,5; Alber, Frank 1,3; Sali, Andrej 8; Larabell, Carolyn 2,5; Stevens, Raymond C.1,4,7
	2020-12
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	6 期号:50
发表状态	已发表
DOI	10.1126/sciadv.abc8262
摘要	Characterizing relationships between cell structures and functions requires mesoscale mapping of intact cells showing subcellular rearrangements following stimulation; however, current approaches are limited in this regard. Here, we report a unique application of soft x-ray tomography to generate three-dimensional reconstructions of whole pancreatic. cells at different time points following glucose-stimulated insulin secretion. Reconstructions following stimulation showed distinct insulin vesicle distribution patterns reflective of altered vesicle pool sizes as they travel through the secretory pathway. Our results show that glucose stimulation caused rapid changes in biochemical composition and/or density of insulin packing, increased mitochondrial volume, and closer proximity of insulin vesicles to mitochondria. Costimulation with exendin-4 (a glucagon-like peptide-1 receptor agonist) prolonged these effects and increased insulin packaging efficiency and vesicle maturation. This study provides unique perspectives on the coordinated structural reorganization and interactions of organelles that dictate cell responses.
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收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	NIH[P41GM103445] ; DOE's Office of Biological and Environmental Research[DE-AC02-5CH11231] ; NIH/NIGMS[R01GM083960][P41GM109824] ; NSFC[31950410543]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000597410300011
出版者	AMER ASSOC ADVANCEMENT SCIENCE
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124909
专题	生命科学与技术学院_硕士生 iHuman研究所_特聘教授组_Andrej Sali组 iHuman研究所_特聘教授组_Raymond Stevens组生命科学与技术学院_博士生
通讯作者	White, Kate L.; Sali, Andrej; Larabell, Carolyn; Stevens, Raymond C.
作者单位	1.Univ Southern Calif, USC Michelson Ctr Convergent Biosci, Bridge Inst, Dept Biol Sci, Los Angeles, CA 90089 USA 2.Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA 3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Inst Quantitat & Computat Biosci, Los Angeles, CA 90095 USA 4.ShanghaiTech Univ, Sch Life Sci & Technol, iHuman Inst, Shanghai 201210, Peoples R China 5.Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA 6.Univ Southern Calif, USC Michelson Ctr Convergent Biosci, Bridge Inst, Dept Comp Sci, Los Angeles, CA 90089 USA 7.Univ Southern Calif, USC Michelson Ctr Convergent Biosci, Bridge Inst, Dept Chem, Los Angeles, CA 90089 USA 8.Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Bioengn & Therapeut Sci, Dept Pharmaceut Chem, San Francisco, CA 94158 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	White, Kate L.,Singla, Jitin,Loconte, Valentina,et al. Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography[J]. SCIENCE ADVANCES,2020,6(50).
APA	White, Kate L..,Singla, Jitin.,Loconte, Valentina.,Chen, Jian-Hua.,Ekman, Axel.,...&Stevens, Raymond C..(2020).Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography.SCIENCE ADVANCES,6(50).
MLA	White, Kate L.,et al."Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography".SCIENCE ADVANCES 6.50(2020).