Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription

doi:10.1186/s13059-020-02201-1

	Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription
	Feng, Ying1,2 ; Wang, Yao 3,4; Wang, Xiangnan5 ; He, Xiaohui5 ; Yang, Chen 6; Naseri, Ardalan 7; Pederson, Thoru 8; Zheng, Jing 6; Zhang, Shaojie 7; Xiao, Xiao 9; Xie, Wei 3,4; Ma, Hanhui5
	2020-12-08
发表期刊	GENOME BIOLOGY (IF:10.1[JCR-2023],16.5[5-Year])
ISSN	1474-760X
卷号	21 期号:1
发表状态	已发表
DOI	10.1186/s13059-020-02201-1
摘要	IntroductionDespite the long-observed correlation between H3K9me3, chromatin architecture, and transcriptional repression, how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear.ResultHere, we develop EpiGo (Epigenetic perturbation induced Genome organization)-KRAB to introduce H3K9me3 at hundreds of loci spanning megabases on human chromosome 19 and simultaneously track genome organization. EpiGo-KRAB is sufficient to induce genomic clustering and de novo heterochromatin-like domain formation, which requires SETDB1, a methyltransferase of H3K9me3. Unexpectedly, EpiGo-KRAB-induced heterochromatin-like domain does not result in widespread gene repression except a small set of genes with concurrent loss of H3K4me3 and H3K27ac. Ectopic H3K9me3 appears to spread in inactive regions but is largely restricted from transcriptional initiation sites in active regions. Finally, Hi-C analysis showed that EpiGo-KRAB reshapes existing compartments mainly at compartment boundaries.ConclusionsThese results reveal the role of H3K9me3 in genome organization could be partially separated from its function in gene repression.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31970591][31988101][31830047][31725018] ; Shanghai Pujiang program[19PJ1408000] ; U.S. National Institutes of Health 4D Nucleome Initiative[U01-DA-040588] ; Beijing Municipal Science & Technology Commission[Z181100001318006]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000600100800002
出版者	BMC
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124908
专题	生命科学与技术学院_硕士生生命科学与技术学院_博士生生命科学与技术学院_PI研究组_马涵慧组
通讯作者	Xie, Wei; Ma, Hanhui
作者单位	1.East China Univ Sci & Technol, Sch Biotechnol, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Stem Cell Biol & Regenerat Med, MOE Key Lab Bioinformat, Beijing, Peoples R China 4.Tsinghua Peking Ctr Life Sci, Beijing, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Beijing, Peoples R China 6.East China Univ Sci & Technol, Sch Pharm, Shanghai, Peoples R China 7.Univ Cent Florida, Dept Comp Sci, Orlando, FL 32816 USA 8.Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA USA 9.East China Univ Sci & Technol, Sch Biotechnol, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Feng, Ying,Wang, Yao,Wang, Xiangnan,et al. Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription[J]. GENOME BIOLOGY,2020,21(1).
APA	Feng, Ying.,Wang, Yao.,Wang, Xiangnan.,He, Xiaohui.,Yang, Chen.,...&Ma, Hanhui.(2020).Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription.GENOME BIOLOGY,21(1).
MLA	Feng, Ying,et al."Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription".GENOME BIOLOGY 21.1(2020).