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ShanghaiTech University Knowledge Management System
Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells | |
2020-11-10 | |
发表期刊 | NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year]) |
ISSN | 2041-1723 |
卷号 | 11期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s41467-020-19414-4 |
摘要 | Clinical efficacy of treatments against non-obstructive azoospermia (NOA), which affects 1% of men, are currently limited by the incomplete understanding of NOA pathogenesis and normal spermatogenic microenvironment. Here, we profile >80,000 human testicular single-cell transcriptomes from 10 healthy donors spanning the range from infant to adult and 7 NOA patients. We show that Sertoli cells, which form the scaffold in the testicular microenvironment, are severely damaged in NOA patients and identify the roadmap of Sertoli cell maturation. Notably, Sertoli cells of patients with congenital causes (Klinefelter syndrome and Y chromosome microdeletions) are mature, but exhibit abnormal immune responses, while the cells in idiopathic NOA (iNOA) are physiologically immature. Furthermore, we find that inhibition of Wnt signaling promotes the maturation of Sertoli cells from iNOA patients, allowing these cells to regain their ability to support germ cell survival. We provide a novel perspective on the development of diagnostic methods and therapeutic targets for NOA. Non-obstructive azoospermia affects 1% of men. Here, authors perform single-cell transcriptomic analysis of human testicular cells from healthy donors and non-obstructive azoospermia patients and find that inhibition of Wnt signaling promotes the maturation of Sertoli cells from patients. |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
资助项目 | National Key R&D Program of China[2017YFC1002003][2018YFA0107702] ; National Natural Science Foundation of China[81671512][81701524][81871215][81701428] ; Doctorial innovation fund of Shanghai Jiao Tong University School of Medicine[BXJ201940] ; China Postdoctoral Science Foundation[2019M661524] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000593978100008 |
出版者 | NATURE RESEARCH |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124685 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_周智组 |
共同第一作者 | Yao, ChenCheng; Xing, XiaoYu; Jing, Tao |
通讯作者 | Sun, Jie; Wang, ChenChen; Zhou, Zhi; Li, Zheng |
作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Shanghai Key Lab Reprod Med,Dept Androl, Urol Med Ctr,Ctr Mens Hlth,Sch Med, Shanghai 200080, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Dept Urol, Shanghai Childrens Med Ctr, Shanghai 200120, Peoples R China 4.Qingdao Univ, Dept Androl, Affiliated Hosp, Qingdao 266000, Shandong, Peoples R China 5.Chinese Acad Sci, Shanghai Adv Res Inst, Stem Cell & Reprod Biol Lab, Shanghai 201210, Peoples R China |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Zhao, LiangYu,Yao, ChenCheng,Xing, XiaoYu,et al. Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells[J]. NATURE COMMUNICATIONS,2020,11(1). |
APA | Zhao, LiangYu.,Yao, ChenCheng.,Xing, XiaoYu.,Jing, Tao.,Li, Peng.,...&Li, Zheng.(2020).Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells.NATURE COMMUNICATIONS,11(1). |
MLA | Zhao, LiangYu,et al."Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells".NATURE COMMUNICATIONS 11.1(2020). |
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