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| Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase | |
| 2020-06 | |
| Source Publication | CATALYSTS
 |
| EISSN | 2073-4344 |
| Volume | 10Issue:6 |
| Status | 已发表 |
| DOI | 10.3390/catal10060663 |
| Abstract | As an important hormone drug intermediate, androst-1,4-diene-3,17-dione can be bio-converted from phytosterols. However, separation and purification in the downstream process are very difficult due to the similarity in structure and physiological characteristics between ADD and androstenedione (AD). This phenomenon was correlated to the insufficient enzyme activity of 3-ketosteroid-Delta(1)-dehydrogenase (KSDD), which specifically catalyzes the C(1,2)dehydrogenation of AD. In order to obtain a highly purified ADD from phytosterols, the dehydrogenation effect of different kinds of KSDDs and the transcription effect of four promoter sequences onksddwere analyzed inMycobacteriumsp. ZFZ (ZFZ), the cell host that transform phytosterols to AD in the oil-aqueous system. A tandem KSDD expression cassette containing strain ZFZ-2111 yielded 2.06 +/- 0.09 g L(-1)ADD, with a molar ratio of ADD/AD at 41.47:1.00 in 120 h. In waste cooking oil-aqueous media, the proportion of ADD in the fermentation by ZFZ-2111 was 92%. The present study provides a reliable theoretical basis for the step-by-step transformation of phytosterols to ADD. |
| Keyword | KSDD Androst-1 4-diene-3 17-dione(ADD) phytosterols waste cooking oil biotransformation |
| URL | 查看原文 |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2017YFE0112700] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Physical |
| WOS ID | WOS:000554688300001 |
| Publisher | MDPI |
| WOS Keyword | GENE-ENCODING 3-KETOSTEROID-DELTA(1)-DEHYDROGENASE ; MICROBIAL CONVERSION ; BETA-CYCLODEXTRIN ; ANDROSTENEDIONE ; IDENTIFICATION ; TRANSFORMATION ; BIOCONVERSION ; MYCOBACTERIA ; CATABOLISM ; STEROLS |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122939 |
| Collection | 生命科学与技术学院_硕士生 生命科学与技术学院_特聘教授组_孙俊松组 生命科学与技术学院_特聘教授组_史吉平组 生命科学与技术学院_博士生 |
| Corresponding Author | Sun, Junsong; Zhang, Baoguo |
| Affiliation | 1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Minnesota, Biotechnol Inst, Minneapolis, MN 55455 USA 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
| Corresponding Author Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Liu, Xiangcen,Zhang, Ruijie,Bao, Zhiwei,et al. Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase[J]. CATALYSTS,2020,10(6). |
| APA | Liu, Xiangcen.,Zhang, Ruijie.,Bao, Zhiwei.,Yuan, Chenyang.,Cao, Huijin.,...&Zhang, Baoguo.(2020).Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase.CATALYSTS,10(6). |
| MLA | Liu, Xiangcen,et al."Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase".CATALYSTS 10.6(2020). |
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