Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase
2020-06
Source PublicationCATALYSTS
EISSN2073-4344
Volume10Issue:6
Status已发表
DOI10.3390/catal10060663
AbstractAs an important hormone drug intermediate, androst-1,4-diene-3,17-dione can be bio-converted from phytosterols. However, separation and purification in the downstream process are very difficult due to the similarity in structure and physiological characteristics between ADD and androstenedione (AD). This phenomenon was correlated to the insufficient enzyme activity of 3-ketosteroid-Delta(1)-dehydrogenase (KSDD), which specifically catalyzes the C(1,2)dehydrogenation of AD. In order to obtain a highly purified ADD from phytosterols, the dehydrogenation effect of different kinds of KSDDs and the transcription effect of four promoter sequences onksddwere analyzed inMycobacteriumsp. ZFZ (ZFZ), the cell host that transform phytosterols to AD in the oil-aqueous system. A tandem KSDD expression cassette containing strain ZFZ-2111 yielded 2.06 +/- 0.09 g L(-1)ADD, with a molar ratio of ADD/AD at 41.47:1.00 in 120 h. In waste cooking oil-aqueous media, the proportion of ADD in the fermentation by ZFZ-2111 was 92%. The present study provides a reliable theoretical basis for the step-by-step transformation of phytosterols to ADD.
KeywordKSDD Androst-1 4-diene-3 17-dione(ADD) phytosterols waste cooking oil biotransformation
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Indexed BySCI ; SCIE
Language英语
Funding ProjectNational Key R&D Program of China[2017YFE0112700]
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000554688300001
PublisherMDPI
WOS KeywordGENE-ENCODING 3-KETOSTEROID-DELTA(1)-DEHYDROGENASE ; MICROBIAL CONVERSION ; BETA-CYCLODEXTRIN ; ANDROSTENEDIONE ; IDENTIFICATION ; TRANSFORMATION ; BIOCONVERSION ; MYCOBACTERIA ; CATABOLISM ; STEROLS
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122939
Collection生命科学与技术学院_硕士生
生命科学与技术学院_特聘教授组_孙俊松组
生命科学与技术学院_特聘教授组_史吉平组
生命科学与技术学院_博士生
Corresponding AuthorSun, Junsong; Zhang, Baoguo
Affiliation
1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Minnesota, Biotechnol Inst, Minneapolis, MN 55455 USA
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Liu, Xiangcen,Zhang, Ruijie,Bao, Zhiwei,et al. Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase[J]. CATALYSTS,2020,10(6).
APA Liu, Xiangcen.,Zhang, Ruijie.,Bao, Zhiwei.,Yuan, Chenyang.,Cao, Huijin.,...&Zhang, Baoguo.(2020).Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase.CATALYSTS,10(6).
MLA Liu, Xiangcen,et al."Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase".CATALYSTS 10.6(2020).
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