Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase

doi:10.3390/catal10060663

	Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase
	Liu, Xiangcen 1,2; Zhang, Ruijie 3; Bao, Zhiwei 1; Yuan, Chenyang1,4 ; Cao, Huijin 1; Shi, Jiping1,2,4 ; Sun, Junsong1,2,4 ; Zhang, Baoguo 1,2
	2020-06
发表期刊	CATALYSTS
EISSN	2073-4344
卷号	10 期号:6
发表状态	已发表
DOI	10.3390/catal10060663
摘要	As an important hormone drug intermediate, androst-1,4-diene-3,17-dione can be bio-converted from phytosterols. However, separation and purification in the downstream process are very difficult due to the similarity in structure and physiological characteristics between ADD and androstenedione (AD). This phenomenon was correlated to the insufficient enzyme activity of 3-ketosteroid-Delta(1)-dehydrogenase (KSDD), which specifically catalyzes the C(1,2)dehydrogenation of AD. In order to obtain a highly purified ADD from phytosterols, the dehydrogenation effect of different kinds of KSDDs and the transcription effect of four promoter sequences onksddwere analyzed inMycobacteriumsp. ZFZ (ZFZ), the cell host that transform phytosterols to AD in the oil-aqueous system. A tandem KSDD expression cassette containing strain ZFZ-2111 yielded 2.06 +/- 0.09 g L(-1)ADD, with a molar ratio of ADD/AD at 41.47:1.00 in 120 h. In waste cooking oil-aqueous media, the proportion of ADD in the fermentation by ZFZ-2111 was 92%. The present study provides a reliable theoretical basis for the step-by-step transformation of phytosterols to ADD.
关键词	KSDD Androst-1 4-diene-3 17-dione(ADD) phytosterols waste cooking oil biotransformation
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Key R&D Program of China[2017YFE0112700]
WOS研究方向	Chemistry
WOS类目	Chemistry, Physical
WOS记录号	WOS:000554688300001
出版者	MDPI
WOS关键词	GENE-ENCODING 3-KETOSTEROID-DELTA(1)-DEHYDROGENASE ; MICROBIAL CONVERSION ; BETA-CYCLODEXTRIN ; ANDROSTENEDIONE ; IDENTIFICATION ; TRANSFORMATION ; BIOCONVERSION ; MYCOBACTERIA ; CATABOLISM ; STEROLS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122939
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_孙俊松组生命科学与技术学院_特聘教授组_史吉平组生命科学与技术学院_博士生
通讯作者	Sun, Junsong; Zhang, Baoguo
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, 99 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Minnesota, Biotechnol Inst, Minneapolis, MN 55455 USA 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Xiangcen,Zhang, Ruijie,Bao, Zhiwei,et al. Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase[J]. CATALYSTS,2020,10(6).
APA	Liu, Xiangcen.,Zhang, Ruijie.,Bao, Zhiwei.,Yuan, Chenyang.,Cao, Huijin.,...&Zhang, Baoguo.(2020).Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase.CATALYSTS,10(6).
MLA	Liu, Xiangcen,et al."Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase".CATALYSTS 10.6(2020).