KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers

doi:10.1007/s00018-020-03570-3

	KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers
	Jia, Jie1,3 ; Yue, Xihua1 ; Zhu, Lianhui1 ; Jing, Shuaiyang1,3 ; Wang, Yijing1,3 ; Gim, Bopil2 ; Qian, Yi1 ; Lee, Intaek1
	2021-02
发表期刊	CELLULAR AND MOLECULAR LIFE SCIENCES (IF:6.2[JCR-2023],7.7[5-Year])
ISSN	1420-682X
EISSN	1420-9071
发表状态	已发表
DOI	10.1007/s00018-020-03570-3
摘要	KDEL receptor cycles between the ER and the Golgi to retrieve ER-resident chaperones that get leaked to the secretory pathway during protein export from the ER. Recent studies have shown that a fraction of KDEL receptor may reside in the plasma membrane and function as a putative cell surface receptor. However, the trafficking itinerary and mechanism of cell surface expressed KDEL receptor remains largely unknown. In this study, we used N-terminally Halo-tagged KDEL receptor to investigate its endocytosis from the plasma membrane and trafficking itinerary of the endocytosed receptor through the endolysosomal compartments. Our results indicate that surface-expressed KDEL receptor undergoes highly complex recycling pathways via the Golgi and peri-nuclear recycling endosomes that are positive for Rab11 and Rab14, respectively. Unexpectedly, KDEL receptor appears to preferentially utilize clathrin-mediated endocytic pathway as well as clathrin-dependent transport carriers for export from thetrans-Golgi network. Taken together, we suggest that KDEL receptor may be a bona fide cell surface receptor with a complex, yet well-defined trafficking itinerary through the endolysosomal compartments.
关键词	KDEL receptor Golgi Rab11 Rab14 Clathrin Membrane trafficking MANF Caveolae EEA1 Lysosome Late endosomes Early endosomes Recycling endosomes
收录类别	SCI ; SCIE
语种	英语
资助项目	Shanghai Pujiang Program[16PJ1407]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000541322600001
出版者	SPRINGER BASEL AG
WOS关键词	GROWTH-FACTOR-II ; ENDOPLASMIC-RETICULUM ; BINDING ; PROTEIN ; MANF ; SPECIFICITY ; RETENTION ; SECRETION ; COMPLEX ; ERD2
原始文献类型	Article ; Early Access
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121804
专题	物质科学与技术学院_PI研究组_yongjin lee组生命科学与技术学院_特聘教授组_James E. Rothman组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Qian, Yi; Lee, Intaek
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Jia, Jie,Yue, Xihua,Zhu, Lianhui,et al. KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2021.
APA	Jia, Jie.,Yue, Xihua.,Zhu, Lianhui.,Jing, Shuaiyang.,Wang, Yijing.,...&Lee, Intaek.(2021).KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers.CELLULAR AND MOLECULAR LIFE SCIENCES.
MLA	Jia, Jie,et al."KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers".CELLULAR AND MOLECULAR LIFE SCIENCES (2021).