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| Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin | |
| 2020-04 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| EISSN | 1422-0067 |
| Volume | 21Issue:8 |
| Status | 已发表 |
| DOI | 10.3390/ijms21082946 |
| Abstract | Tooth enamel is formed in an extracellular environment. Amelogenin, the major component in the protein matrix of tooth enamel during the developing stage, could assemble into high molecular weight structures, regulating enamel formation. However, the molecular structure of amelogenin protein assembly at the functional state is still elusive. In this work, we found that amelogenin is able to induce calcium phosphate minerals into hydroxyapatite (HAP) structure in vitro at pH 6.0. Assessed using X-ray diffraction (XRD) and P-31 solid-state NMR (SSNMR) evidence, the formed HAP mimics natural enamel closely. The structure of amelogenin protein assembly coexisting with the HAP was also studied using atomic force microscopy (AFM), transmission electron microscopy (TEM) and XRD, indicating the beta-amyloid structure of the protein. SSNMR was proven to be an important tool in detecting both the rigid and dynamic components of the protein assembly in the sample, and the core sequence (18)EVLTPLKWYQSI(29) was identified as the major segment contributing to the beta-sheet secondary structure. Our research suggests an amyloid structure may be an important factor in controlling HAP formation at the right pH conditions with the help of other structural components in the protein assembly. |
| Keyword | amelogenin hydroxyapatite enamel biomimetic protein assembly structure solid-state NMR |
| URL | 查看原文 |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | National Science Foundation of China[31770790] ; Yangfan program of Shanghai municipal government[19YF1433500] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS ID | WOS:000535565300288 |
| Publisher | MDPI |
| WOS Keyword | SOLID-STATE NMR ; CALCIUM PHOSPHATES ; ENAMEL ; ASSIGNMENT ; BACKBONE ; SPECTROSCOPY ; IMPERFECTA ; PROTEINS |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121626 |
| Collection | 生命科学与技术学院_硕士生 生命科学与技术学院_PI研究组_陆珺霞组 生命科学与技术学院_公共科研平台_分子细胞学平台 生命科学与技术学院_博士生 |
| Corresponding Author | Lu, Junxia |
| Affiliation | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | School of Life Science and Technology |
| Corresponding Author Affilication | School of Life Science and Technology |
| First Signature Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Zhang, Jing,Wang, Jian,Ma, Chengwei,et al. Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(8). |
| APA | Zhang, Jing,Wang, Jian,Ma, Chengwei,&Lu, Junxia.(2020).Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(8). |
| MLA | Zhang, Jing,et al."Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.8(2020). |
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