Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin

doi:10.3390/ijms21082946

	Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin
	Zhang, Jing1,2,3 ; Wang, Jian1 ; Ma, Chengwei1 ; Lu, Junxia1
	2020-04
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
卷号	21 期号:8
发表状态	已发表
DOI	10.3390/ijms21082946
摘要	Tooth enamel is formed in an extracellular environment. Amelogenin, the major component in the protein matrix of tooth enamel during the developing stage, could assemble into high molecular weight structures, regulating enamel formation. However, the molecular structure of amelogenin protein assembly at the functional state is still elusive. In this work, we found that amelogenin is able to induce calcium phosphate minerals into hydroxyapatite (HAP) structure in vitro at pH 6.0. Assessed using X-ray diffraction (XRD) and P-31 solid-state NMR (SSNMR) evidence, the formed HAP mimics natural enamel closely. The structure of amelogenin protein assembly coexisting with the HAP was also studied using atomic force microscopy (AFM), transmission electron microscopy (TEM) and XRD, indicating the beta-amyloid structure of the protein. SSNMR was proven to be an important tool in detecting both the rigid and dynamic components of the protein assembly in the sample, and the core sequence (18)EVLTPLKWYQSI(29) was identified as the major segment contributing to the beta-sheet secondary structure. Our research suggests an amyloid structure may be an important factor in controlling HAP formation at the right pH conditions with the help of other structural components in the protein assembly.
关键词	amelogenin hydroxyapatite enamel biomimetic protein assembly structure solid-state NMR
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Science Foundation of China[31770790] ; Yangfan program of Shanghai municipal government[19YF1433500]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000535565300288
出版者	MDPI
WOS关键词	SOLID-STATE NMR ; CALCIUM PHOSPHATES ; ENAMEL ; ASSIGNMENT ; BACKBONE ; SPECTROSCOPY ; IMPERFECTA ; PROTEINS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121626
专题	生命科学与技术学院_硕士生生命科学与技术学院_PI研究组_陆珺霞组生命科学与技术学院_公共科研平台_分子细胞学平台生命科学与技术学院_博士生
通讯作者	Lu, Junxia
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Jing,Wang, Jian,Ma, Chengwei,et al. Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(8).
APA	Zhang, Jing,Wang, Jian,Ma, Chengwei,&Lu, Junxia.(2020).Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(8).
MLA	Zhang, Jing,et al."Hydroxyapatite Formation Coexists with Amyloid-like Self-Assembly of Human Amelogenin".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.8(2020).