Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy

doi:10.1002/1873-3468.13719

	Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy
	Liu, Yin 1; Li, Guanglei2 ; Yang, Guang2 ; Gu, Huifeng 1; Huang, Shisheng2 ; Yu, Wenxia2 ; Qin, Guizhen 1; Liu, Xinyi 3; Zhou, Fuling 4; Huang, Xingxu2 ; Wei, Yongchang 1
	2019-12-29
发表期刊	FEBS LETTERS (IF:3.0[JCR-2023],3.8[5-Year])
ISSN	0014-5793
EISSN	1873-3468
发表状态	已发表
DOI	10.1002/1873-3468.13719
摘要	Base editors (BEs) are widely used in precise gene editing due to their simplicity and versatility. However, their efficiencies are hindered by various obstacles. Considering the chromatin microenvironment as a possible obstacle, here, we demonstrate a further development of the proxy-clustered regularly interspaced short palindromic repeats strategy, termed Proxy-BE, to increase gene editing efficiency. Specifically, a nuclease-dead Cas9 (dCas9) was bound to the sequence about 20-30 base pair away from the target site, potentially improving access to the DNA and, thus, providing a better editing microenvironment for base editors. Our findings confirm that nuclease-dead Streptococcus pyogenes Cas9 can assist the base editors SaKKH-BE3 and dCpf1-BE to double their canonical base editing efficiency. This work provides a new approach to enhance base editing, extending its scope for biological research and gene therapy.
关键词	base editing Cas9 CRISPR dCpf1-BE gene editing SaKKH-BE3
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[81673033]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000504625200001
出版者	WILEY
WOS关键词	GENOMIC DNA ; MOUSE MODEL ; CRISPR-CAS9 ; MUSCLE ; CAS9
原始文献类型	Article ; Early Access
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104977
专题	生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_硕士生
通讯作者	Wei, Yongchang
作者单位	1.Wuhan Univ, Zhongnan Hosp, Dept Radiat & Med Oncol, Wuhan 430071, Hubei, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Gastroenterol, Shenzhen, Guangdong, Peoples R China 4.Wuhan Univ, Zhongnan Hosp, Dept Hematol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yin,Li, Guanglei,Yang, Guang,et al. Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy[J]. FEBS LETTERS,2019.
APA	Liu, Yin.,Li, Guanglei.,Yang, Guang.,Gu, Huifeng.,Huang, Shisheng.,...&Wei, Yongchang.(2019).Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy.FEBS LETTERS.
MLA	Liu, Yin,et al."Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy".FEBS LETTERS (2019).