Requirement for CCNB1 in mouse spermatogenesis

doi:10.1038/cddis.2017.555

	Requirement for CCNB1 in mouse spermatogenesis
	Tang, Ji-Xin 1,2; Li, Jian 1,2; Cheng, Jin-Mei 1,2; Hu, Bian 3,4; Sun, Tie-Cheng 1,2; Li, Xiao-Yu 1,2; Batool, Aalia 1,2; Wang, Zhi-Peng 1,2; Wang, Xiu-Xia 1; Deng, Shou-Long 1; Zhang, Yan 1; Chen, Su-Ren 1; Huang, Xingxu3,4 ; Liu, Yi-Xun 1,2
	2017-10
发表期刊	CELL DEATH & DISEASE (IF:8.1[JCR-2023],8.6[5-Year])
ISSN	2041-4889
卷号	8
发表状态	已发表
DOI	10.1038/cddis.2017.555
摘要	Spermatogenesis, which involves mitosis and meiosis of male germ cells, is a highly complicated and coordinately ordered process. Cyclin B1 (CCNB1), an important regulator in cell cycle machinery, is proved essential for mouse embryonic development. However, the role of CCNB1 in mammalian spermatogenesis remains unclear. Here we tested the requirement for CCNB1 using conditional knockout mice lacking CCNB1 in male germ cells. We found that ablation of CCNB1 in gonocytes and spermatogonia led to mouse sterile caused by the male germ cells' depletion. Gonocyte and spermatogonia without CCNB1 is unable to proliferate normally and apoptosis increased. Moreover, CCNB1 ablation in spermatogonia may promote their differentiation by downregulating Lin28a and upregulating let-7 miRNA. However, ablation of CCNB1 in premeiotic male germ cells did not have an effect on meiosis of spermatocytes and male fertility, suggesting that CCNB1 may be dispensable for meiosis of spermatocytes. Collectively, these results indicate that CCNB1 is critically required for the proliferation of gonocytes and spermatogonia but may be redundant in meiosis of spermatocytes in mouse spermatogenesis.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31501953] ; National Natural Science Foundation of China[31501161] ; National Natural Science Foundation of China[31471352] ; National Natural Science Foundation of China[31471400] ; National Natural Science Foundation of China[81270662] ; National Natural Science Foundation of China[31171380]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000414022900017
出版者	NATURE PUBLISHING GROUP
WOS关键词	MATURATION-PROMOTING FACTOR ; GERMINAL VESICLE BREAKDOWN ; CDC2 PROTEIN-KINASE ; CONTROL GENE CDC2+ ; CELL-CYCLE ; CYTOPLASMIC FACTOR ; M-PHASE ; MEIOTIC MATURATION ; XENOPUS-LAEVIS ; KIT EXPRESSION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9911
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Liu, Yi-Xun
作者单位	1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, 1 Beichen West Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Tang, Ji-Xin,Li, Jian,Cheng, Jin-Mei,et al. Requirement for CCNB1 in mouse spermatogenesis[J]. CELL DEATH & DISEASE,2017,8.
APA	Tang, Ji-Xin.,Li, Jian.,Cheng, Jin-Mei.,Hu, Bian.,Sun, Tie-Cheng.,...&Liu, Yi-Xun.(2017).Requirement for CCNB1 in mouse spermatogenesis.CELL DEATH & DISEASE,8.
MLA	Tang, Ji-Xin,et al."Requirement for CCNB1 in mouse spermatogenesis".CELL DEATH & DISEASE 8(2017).