Requirement for CCNB1 in mouse spermatogenesis
Tang, Ji-Xin1,2; Li, Jian1,2; Cheng, Jin-Mei1,2; Hu, Bian3,4; Sun, Tie-Cheng1,2; Li, Xiao-Yu1,2; Batool, Aalia1,2; Wang, Zhi-Peng1,2; Wang, Xiu-Xia1; Deng, Shou-Long1
2017-10
发表期刊CELL DEATH & DISEASE
ISSN2041-4889
卷号8
发表状态已发表
DOI10.1038/cddis.2017.555
摘要Spermatogenesis, which involves mitosis and meiosis of male germ cells, is a highly complicated and coordinately ordered process. Cyclin B1 (CCNB1), an important regulator in cell cycle machinery, is proved essential for mouse embryonic development. However, the role of CCNB1 in mammalian spermatogenesis remains unclear. Here we tested the requirement for CCNB1 using conditional knockout mice lacking CCNB1 in male germ cells. We found that ablation of CCNB1 in gonocytes and spermatogonia led to mouse sterile caused by the male germ cells' depletion. Gonocyte and spermatogonia without CCNB1 is unable to proliferate normally and apoptosis increased. Moreover, CCNB1 ablation in spermatogonia may promote their differentiation by downregulating Lin28a and upregulating let-7 miRNA. However, ablation of CCNB1 in premeiotic male germ cells did not have an effect on meiosis of spermatocytes and male fertility, suggesting that CCNB1 may be dispensable for meiosis of spermatocytes. Collectively, these results indicate that CCNB1 is critically required for the proliferation of gonocytes and spermatogonia but may be redundant in meiosis of spermatocytes in mouse spermatogenesis.
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31501953] ; National Natural Science Foundation of China[31501161] ; National Natural Science Foundation of China[31471352] ; National Natural Science Foundation of China[31471400] ; National Natural Science Foundation of China[81270662] ; National Natural Science Foundation of China[31171380]
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000414022900017
出版者NATURE PUBLISHING GROUP
WOS关键词MATURATION-PROMOTING FACTOR ; GERMINAL VESICLE BREAKDOWN ; CDC2 PROTEIN-KINASE ; CONTROL GENE CDC2+ ; CELL-CYCLE ; CYTOPLASMIC FACTOR ; M-PHASE ; MEIOTIC MATURATION ; XENOPUS-LAEVIS ; KIT EXPRESSION
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9911
专题生命科学与技术学院_PI研究组_黄行许组
通讯作者Liu, Yi-Xun
作者单位1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, 1 Beichen West Rd, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
4.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China
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GB/T 7714
Tang, Ji-Xin,Li, Jian,Cheng, Jin-Mei,et al. Requirement for CCNB1 in mouse spermatogenesis[J]. CELL DEATH & DISEASE,2017,8.
APA Tang, Ji-Xin.,Li, Jian.,Cheng, Jin-Mei.,Hu, Bian.,Sun, Tie-Cheng.,...&Liu, Yi-Xun.(2017).Requirement for CCNB1 in mouse spermatogenesis.CELL DEATH & DISEASE,8.
MLA Tang, Ji-Xin,et al."Requirement for CCNB1 in mouse spermatogenesis".CELL DEATH & DISEASE 8(2017).
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