Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry

doi:10.1039/c7ay01891f

	Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry
	Ma, Jun 1,5; Lu, Yan1,2 ; Wu, Dong1 ; Peng, Yao1,2,3 ; Loa-Kum-Cheung, Wendy 4; Peng, Chao 5; Quinn, Ronald J.4; Shui, Wenqing1,2 ; Liu, Zhi-Jie1,2,3
	2017-10-28
发表期刊	ANALYTICAL METHODS (IF:2.7[JCR-2023],2.7[5-Year])
ISSN	1759-9660
卷号	9 期号:40 页码:5851-5858
发表状态	已发表
DOI	10.1039/c7ay01891f
摘要	A nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A(2A) receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively stable, unliganded/apo and native state.
收录类别	SCI ; EI
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS记录号	WOS:000413289200003
出版者	ROYAL SOC CHEMISTRY
EI入藏号	20174304299987
EI主题词	Binding energy ; Drug products ; Mass spectrometry ; Phospholipids ; Spectrometry
EI分类号	Chemistry:801 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Optical Variables Measurements:941.4
WOS关键词	PROTEIN-COUPLED RECEPTORS ; MEMBRANE-PROTEINS ; IN-VITRO ; INHIBITION ; RHODOPSIN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9901
专题	生命科学与技术学院_博士生 iHuman研究所_特聘教授组_Raymond Stevens组 iHuman研究所_PI研究组_刘志杰组 iHuman研究所_PI研究组_陶厚朝组 iHuman研究所_PI研究组_水雯箐组
通讯作者	Quinn, Ronald J.; Shui, Wenqing; Liu, Zhi-Jie
作者单位	1.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 3.Kunming Med Univ, Inst Mol & Clin Med, Kunming 650500, Yunnan, Peoples R China 4.Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld 4111, Australia 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, Shanghai, Peoples R China
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所; 生命科学与技术学院
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Ma, Jun,Lu, Yan,Wu, Dong,et al. Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry[J]. ANALYTICAL METHODS,2017,9(40):5851-5858.
APA	Ma, Jun.,Lu, Yan.,Wu, Dong.,Peng, Yao.,Loa-Kum-Cheung, Wendy.,...&Liu, Zhi-Jie.(2017).Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry.ANALYTICAL METHODS,9(40),5851-5858.
MLA	Ma, Jun,et al."Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry".ANALYTICAL METHODS 9.40(2017):5851-5858.