Temporal regulation of prenatal embryonic development by paternal imprinted loci

doi:10.1007/s11427-019-9817-6

	Temporal regulation of prenatal embryonic development by paternal imprinted loci
	Li, Qing 1; Li, Yuanyuan 1; Yin, Qi 1; Huang, Shuo2 ; Wang, Kai 1; Zhuo, Liangchai2 ; Li, Wei 1; Chang, Boran 1; Li, Jinsong1,2
	2020-01
发表期刊	SCIENCE CHINA-LIFE SCIENCES (IF:8.0[JCR-2023],7.3[5-Year])
ISSN	1674-7305
EISSN	1869-1889
卷号	63 期号:1 页码:1-17
发表状态	已发表
DOI	10.1007/s11427-019-9817-6
摘要	Paternal imprinted genes (H19 and Gtl2) are pivotal for prenatal embryonic development in mice. Nongrowing oocytes and sperm- or oocyte-originated haploid embryonic stem cells (haESCs) carrying both H19-DMR (differentially DNA-methylated region) and IG (intergenic)-DMR deletions that partially mimic paternal imprinting of H19-Igf2 and Dlk1-Dio3 can be employed as sperm replacement to efficiently support full-term embryonic development. However, how H19-DMR and IG-DMR act together to regulate embryonic development is still largely unknown. Here, using androgenetic haESC (AG-haESC)-mediated semi-cloned (SC) technology, we showed that paternal H19-DMR and IG-DMR are not essential for pre-implantation development of SC embryos generated through injection of AG-haESCs into oocytes. H19-DMR plays critical roles before 12.5 days of gestation while IG-DMR is essential for late-gestation of SC embryos. Interestingly, we found that combined deletions of H19 and H19-DMR can further improve the efficiency of normal development of SC embryos at mid-gestation compared to DKO SC embryos. Transcriptome and histology analyses revealed that H19 and H19-DMR combined deletions rescue the placental defects. Furthermore, we showed that H19, H19-DMR and IG-DMR deletions (TKO) give rise to better prenatal and postnatal embryonic development of SC embryos compared to DKO. Together, our results indicate the temporal regulation of paternal imprinted loci during embryonic development.
关键词	imprinted loci semi-cloned technology temporal regulation H19-Igf2 Dlk1-Dio3 embryonic development
收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000511500100001
出版者	SCIENCE PRESS
WOS关键词	ENHANCER-BLOCKING ACTIVITY ; H19 GENE ; IGF-II ; MOUSE ; MICE ; CELLS ; EXPRESSION ; GENERATION ; METHYLATION ; PROTEIN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/67986
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_李劲松组
通讯作者	Li, Jinsong
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab C, Shanghai Key Lab Mol Androl,CAS Ctr Excellence Mo, Shanghai 200031, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Qing,Li, Yuanyuan,Yin, Qi,et al. Temporal regulation of prenatal embryonic development by paternal imprinted loci[J]. SCIENCE CHINA-LIFE SCIENCES,2020,63(1):1-17.
APA	Li, Qing.,Li, Yuanyuan.,Yin, Qi.,Huang, Shuo.,Wang, Kai.,...&Li, Jinsong.(2020).Temporal regulation of prenatal embryonic development by paternal imprinted loci.SCIENCE CHINA-LIFE SCIENCES,63(1),1-17.
MLA	Li, Qing,et al."Temporal regulation of prenatal embryonic development by paternal imprinted loci".SCIENCE CHINA-LIFE SCIENCES 63.1(2020):1-17.