Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

doi:10.1186/s12934-019-1183-0

	Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method
	Zhang, Xian 1,2; Han, Rumeng 1; Bao, Teng 2; Zhao, Xiaojing3 ; Li, Xiangfei 1; Zhu, Manchi 1; Yang, Taowei 1; Xu, Meijuan 1; Shao, Minglong 1; Zhao, Youxi 4; Rao, Zhiming 1
	2019-08-06
发表期刊	MICROBIAL CELL FACTORIES
卷号	18 期号:1
发表状态	已发表
DOI	10.1186/s12934-019-1183-0
摘要	Background Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion and mutually transition between AC and 2,3-BD in their natural producing strains not only led to a low selectivity but also increase the difficulty of downstream purification. Therefore, synthetic engineering of more suitable strains should be a reliable strategy to selectively produce AC and 2,3-BD, respectively. Results In this study, the respective AC (alsS and alsD) and 2,3-BD biosynthesis pathway genes (alsS, alsD, and bdhA) derived from Bacillus subtilis 168 were successfully expressed in non-natural AC and 2,3-BD producing Corynebacterium crenatum, and generated recombinant strains, C. crenatum SD and C. crenatum SDA, were proved to produce 9.86 g L-1 of AC and 17.08 g L-1 of 2,3-BD, respectively. To further increase AC and 2,3-BD selectivity, the AC reducing gene (butA) and lactic acid dehydrogenase gene (ldh) in C. crenatum were then deleted. Finally, C. crenatum Delta butA Delta ldh SD produced 76.93 g L-1 AC in one-step biocatalysis with the yield of 0.67 mol mol(-1). Meanwhile, after eliminating the lactic acid production and enhancing 2,3-butanediol dehydrogenase activity, C. crenatum Delta ldh SDA synthesized 88.83 g L-1 of 2,3-BD with the yield of 0.80 mol mol(-1). Conclusions The synthetically engineered C. crenatum Delta butA Delta ldh SD and C. crenatum Delta ldh SDA in this study were proved as an efficient microbial cell factory for selective AC and 2,3-BD production. Based on the insights from this study, further synthetic engineering of C. crenatum for AC and 2,3-BD production is suggested.
关键词	Corynebacterium crenatum Synthetic engineering Biocatalysis Acetoin 2 3-Butanediol
收录类别	SCI ; SCIE
语种	英语
资助项目	National First-class Discipline Program of Light Industry Technology and Engineering[LITE2018-06]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000479067800001
出版者	BMC
WOS关键词	WHOLE-CELL BIOCATALYST ; SACCHAROMYCES-CEREVISIAE ; BACILLUS-SUBTILIS ; ACID PRODUCTION ; SUCCINIC ACID ; DEHYDROGENASE ; EXPRESSION ; REDUCTASE ; GENE ; (2S,3S)-2,3-BUTANEDIOL
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/64586
专题	生命科学与技术学院_PI研究组_张力烨组
通讯作者	Rao, Zhiming
作者单位	1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China 2.Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA 3.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 4.Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 10023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xian,Han, Rumeng,Bao, Teng,et al. Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method[J]. MICROBIAL CELL FACTORIES,2019,18(1).
APA	Zhang, Xian.,Han, Rumeng.,Bao, Teng.,Zhao, Xiaojing.,Li, Xiangfei.,...&Rao, Zhiming.(2019).Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.MICROBIAL CELL FACTORIES,18(1).
MLA	Zhang, Xian,et al."Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method".MICROBIAL CELL FACTORIES 18.1(2019).