Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human

doi:10.3389/fphys.2019.00765

	Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human
	Gao, Huihui 1; Wen, Hui 2; Cao, Congcong 2; Dong, Daqian 1; Yang, Chenhao 1; Xie, Shengsong 3,4; Zhang, Jin 1,2; Huang, Xunbin 2,5; Huang, Xingxu6 ; Yuan, Shuiqiao 2,7; Dong, Wuzi 1
	2019-06-18
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	10
发表状态	已发表
DOI	10.3389/fphys.2019.00765
摘要	Spermatogenesis is a complicated process including spermatogonial stem cells self-renewal and differentiates into mature spermatozoa. MicroRNAs (miRNAs) as a class of small non-coding RNAs play a crucial role during the process of spermatogenesis. However, the function of a plenty of miRNAs on spermatogenesis and the potential mechanisms remain largely unknown. Here, we show that genetically conditional overexpressed miR-10a in germ cells caused complete male sterility, characterized by meiotic arrested in germ cells. Analysis of miR-10a overexpression mouse testes reveals that failure of double strand break (DSB) repairs and aberrant spermatogonial differentiation. Furthermore, we identified Rad51 as a key target of miR-10a in germ cell by bioinformatics prediction and luciferase assay, which may be responsible for the infertility of the miR-10a overexpressed mice and germ cell arrested patients. Our data show that miR-10a dependent genetic regulation of meiotic process is crucial for male germ cell development and spermatogenesis in both mouse and human. These findings facilitate our understanding of the roles of miRNA-10a in spermatogenesis and male fertility.
关键词	miRNA-10a spermatogenesis overexpression mice Rad51 meiosis non-obstructive azoospermia
收录类别	SCI ; SCIE
语种	英语
资助项目	Science, Technology and Innovation Commission of Shenzhen Municipality[JCYJ20170244]
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000472050900002
出版者	FRONTIERS MEDIA SA
WOS关键词	RETINOIC ACID ; MIR-10A ; PROTEINS ; SPERMATOGENESIS ; DIFFERENTIATION ; CONTRIBUTES ; GENERATION ; MIR-34B/C ; RENEWAL ; CORE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/49489
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Yuan, Shuiqiao; Dong, Wuzi
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Inst Reprod Hlth, Wuhan, Hubei, Peoples R China 3.Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan, Hubei, Peoples R China 4.Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan, Hubei, Peoples R China 5.Wuhan Tongji Reprod Med Hosp, Wuhan, Hubei, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 7.Shenzhen Huazhong Univ Sci & Technol Res Inst, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Gao, Huihui,Wen, Hui,Cao, Congcong,et al. Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human[J]. FRONTIERS IN PHYSIOLOGY,2019,10.
APA	Gao, Huihui.,Wen, Hui.,Cao, Congcong.,Dong, Daqian.,Yang, Chenhao.,...&Dong, Wuzi.(2019).Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human.FRONTIERS IN PHYSIOLOGY,10.
MLA	Gao, Huihui,et al."Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human".FRONTIERS IN PHYSIOLOGY 10(2019).