Engineered Living Glues for Autonomous Detection and On-Demand Treatment of Inflammatory Bowel Disease

doi:10.1101/2025.01.04.630840

	Engineered Living Glues for Autonomous Detection and On-Demand Treatment of Inflammatory Bowel Disease
	Ge, Changhao 1; Jiang, Shanshan ; Dong, Xiaomin 1; Jiang, Xiaoyu3 ; Zhi, Weiliang4 ; Xiang, Yunqing 1; Yang, Peilang 5; Zhang, Qian 1; Chen, Xin 2; Liu, Yan 5; Huang, Shuqiang 1; Liu, Yifan3 ; Lin, Jing ; An, Bolin 1; Huang, Peng 2; Zhong, Chao 1
	2025-01-04
状态	已发表
摘要	Smart biomaterials capable of autonomously sensing pathological conditions and executing targeted medical interventions offer therapeutic advantages over conventional passive biomaterials. However, their development remains a considerable challenge. Here, we introduce a therapeutic “living glue” for the automatic detection and on-demand treatment of inflammatory bowel disease (IBD). This living glue employs a genetically engineered, non-pathogenic Escherichia coli with a highly sensitive blood-inducible gene circuit to monitor gastrointestinal bleeding, a key indicator of severe IBD. Upon detection, the bacteria respond by producing potent adhesive and therapeutic proteins around bleeding sites, enabling robust attachment to inflamed tissues and sustained treatment. In a dextran sulfate sodium (DSS)-induced mouse model, a single rectal administration of the living glue markedly improved weight recovery, reversed colonic shortening, and reduced intestinal bleeding. Additionally, the living glue decreased intestinal inflammation, promoted mucosal repair, and restored gut barrier integrity, demonstrating comprehensive therapeutic effects in alleviating IBD symptoms. This study highlights the potential of integrating programmable, living components into biomaterials for autonomous, targeted, and enhanced medical interventions.
语种	英语
DOI	10.1101/2025.01.04.630840
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:120299012
WOS类目	Biology
资助项目	China Postdoctoral Science Foundation["2024T170582","2024M752123"] ; National Key R&D Program of China["2020YFA0908100","2020YFA0908800"] ; National Science Fund for Distinguished Young Scholars[32125023] ; Shenzhen Science and Technology Program["ZDSYS20220606100606013","KQTD20190929172538530"] ; National Natural Science Foundation of China["32201105","32401222"] ; Shenzhen Medical Research Fund["A2303072","B2302047"] ; Postdoctoral Fellowship Program of CPSF[GZC20231714]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/490280
专题	物质科学与技术学院生命科学与技术学院生命科学与技术学院_博士生物质科学与技术学院_博士生物质科学与技术学院_PI研究组_刘一凡组
通讯作者	An, Bolin; Huang, Peng; Zhong, Chao
作者单位	1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Ctr Mat Synthet Biol,Key Lab Quantitat Synthet Biol, Shenzhen 518055, Peoples R China 2.Shenzhen Univ, Int Canc Ctr, Sch Biomed Engn, Marshall Lab Biomed Engn,Lab Evolutionary Theranost LET,Hlth Sci Ctr, Shenzhen 518060, Peoples R China 3.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Shanghai Jiao Tong Univ, Dept Burn, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China
推荐引用方式 GB/T 7714	Ge, Changhao,Jiang, Shanshan,Dong, Xiaomin,et al. Engineered Living Glues for Autonomous Detection and On-Demand Treatment of Inflammatory Bowel Disease. 2025.