Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme

doi:10.1038/s41467-019-10496-3

	Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme
	Wan, Futang1,2,3 ; Wang, Qianmin 4,5; Tan, Jing 1,2; Tan, Ming 1,2; Chen, Juan 4,5; Shi, Shaohua 4,5; Lan, Pengfei 4,5; Wu, Jian 4,5,6; Lei, Ming 4,5,7
	2019-06-13
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	10
发表状态	已发表
DOI	10.1038/s41467-019-10496-3
摘要	Ribonuclease P (RNase P) is an essential ribozyme responsible for tRNA 5' maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii (Mja) RNase P holoenzyme alone and in complex with a tRNA substrate at resolutions of 4.6 angstrom and 4.3 angstrom, respectively. The structures reveal that the subunits of MjaRNase P are strung together to organize the holoenzyme in a dimeric conformation required for efficient catalysis. The structures also show that archaeal RNase P is a functional chimera of bacterial and eukaryal RNase Ps that possesses bacterial-like two RNA-based anchors and a eukaryal-like protein-aided stabilization mechanism. The 3'-RCCA sequence of tRNA, which is a key recognition element for bacterial RNase P, is dispensable for tRNA recognition by MjaRNase P. The overall organization of MjaRNase P, particularly within the active site, is similar to those of bacterial and eukaryal RNase Ps, suggesting a universal catalytic mechanism for all RNase Ps.
收录类别	SCI ; SCIE
语种	英语
资助项目	Young Elite Scientist Sponsorship Program of China Association for Science and Technology[2018QNRC001]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000471227300020
出版者	NATURE PUBLISHING GROUP
WOS关键词	PYROCOCCUS-HORIKOSHII OT3 ; BACTERIAL RNASE-P ; CRYSTAL-STRUCTURE ; METAL-ION ; SUBSTRATE RECOGNITION ; PROTEIN COFACTORS ; C5 PROTEIN ; M1 RNA ; MODEL ; RIBOZYME
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/48980
专题	生命科学与技术学院_博士生
通讯作者	Lan, Pengfei; Wu, Jian; Lei, Ming
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai 200125, Peoples R China 5.Shanghai Inst Precis Med, Shanghai 200125, Peoples R China 6.Shanghai Key Lab Translat Med Ear & Nose Dis, Shanghai 200125, Peoples R China 7.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wan, Futang,Wang, Qianmin,Tan, Jing,et al. Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme[J]. NATURE COMMUNICATIONS,2019,10.
APA	Wan, Futang.,Wang, Qianmin.,Tan, Jing.,Tan, Ming.,Chen, Juan.,...&Lei, Ming.(2019).Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme.NATURE COMMUNICATIONS,10.
MLA	Wan, Futang,et al."Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme".NATURE COMMUNICATIONS 10(2019).