Essential Function of Transmembrane Transcription Factor MYRF in Promoting Transcription of miRNA <italic>lin</italic>-4 during <italic>C. elegans</italic> Development

doi:10.1101/2023.06.22.546200

	Essential Function of Transmembrane Transcription Factor MYRF in Promoting Transcription of miRNA lin-4 during C. elegans Development
	Xu, Zhimin; Wang, Zhao; Wang, Lifang; Qi, Yingchuan B.
	2024-03-03
状态	已发表
摘要	Precise developmental timing control is essential for organism formation and function, but its mechanisms are unclear. In C. elegans, the microRNA lin-4 critically regulates developmental timing by post-transcriptionally downregulating the larval-stage-fate controller LIN-14. However, the mechanisms triggering the activation of lin-4 expression toward the end of the first larval stage remain unknown. We demonstrate that the transmembrane transcription factor MYRF-1 is necessary for lin-4 activation. MYRF-1 is initially localized on the cell membrane, and its increased cleavage and nuclear accumulation coincide with lin-4 expression timing. MYRF-1 regulates lin-4 expression cell-autonomously and hyperactive MYRF-1 can prematurely drive lin-4 expression in embryos and young first-stage larvae. The tandem lin-4 promoter DNA recruits MYRF-1^GFP to form visible loci in the nucleus, suggesting that MYRF-1 directly binds to the lin-4 promoter. Our findings identify a crucial link in understanding developmental timing regulation and establish MYRF-1 as a key regulator of lin-4 expression.
语种	英语
DOI	10.1101/2023.06.22.546200
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:87991555
WOS类目	Developmental Biology
资助项目	National Science Foundation for Young Scientists of China[31900397] ; NIH Office of Research Infrastructure Programs[P40 OD010440]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/446062
专题	生命科学与技术学院生命科学与技术学院_PI研究组_齐瀛川组生命科学与技术学院_博士生
通讯作者	Qi, Yingchuan B.
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhimin,Wang, Zhao,Wang, Lifang,et al. Essential Function of Transmembrane Transcription Factor MYRF in Promoting Transcription of miRNA lin-4 during C. elegans Development. 2024.