An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo

doi:doi.org/10.1073/pnas.2207210120

	An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo
	Kuo Liu 1,2; Muxue Tang 1; Wei Xu 3; Xinfeng Meng2,4 ; Hengwei Jin 2; Maoying Han2,4 ; Jing Pu 3; Yutang Li 3; Fanke Jiao 3; Ruilin Sunhttps://orcid.org/0009-0006-5481-6015 5; Ruling Shen 6; Kathy O. Lui 7; Lu Luhttps://orcid.org/0000-0002-2255-0391 3; Bin Zhou1,2,4
	2023-07-12
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
卷号	120 期号:25 页码:e2207210120
发表状态	已发表
DOI	doi.org/10.1073/pnas.2207210120
摘要	The classical manifestation of COVID-19 is pulmonary infection. After host cell entry via human angiotensin–converting enzyme II (hACE2), the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus can infect pulmonary epithelial cells, especially the AT2 (alveolar type II) cells that are crucial for maintaining normal lung function. However, previous hACE2 transgenic models have failed to specifically and efficiently target the cell types that express hACE2 in humans, especially AT2 cells. In this study, we report an inducible, transgenic hACE2 mouse line and showcase three examples for specifically expressing hACE2 in three different lung epithelial cells, including AT2 cells, club cells, and ciliated cells. Moreover, all these mice models develop severe pneumonia after SARS-CoV-2 infection. This study demonstrates that the hACE2 model can be used to precisely study any cell type of interest with regard to COVID-19-related pathologies.
关键词	hACE2 transgenic mode COVID-19 SARS-CoV-2 pneumonia lineage tracing
语种	英语
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/421409
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_周斌组
共同第一作者	Kuo Liu; Maoying Han
通讯作者	Lu Luhttps://orcid.org/0000-0002-2255-0391; Bin Zhou
作者单位	1.Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, 310024 Hangzhou, China 2.New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 200031 Shanghai, China 3.Key Laboratory of Medical Molecular Virology, Ministry of Education/National Health Commission/Chinese Academy of Medical Science, Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, Fudan University, 200032 Shanghai, China 4.School of Life Science and Technology, ShanghaiTech University, 201210 Shanghai, China 5.Shanghai Engineering Research Center for model organizations, Shanghai Model Organisms Center, Inc., 201318 Shanghai, China 6.Shanghai Laboratory Animal Research Center, 201203 Shanghai, China 7.Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, 999077 Hong Kong, China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Kuo Liu,Muxue Tang,Wei Xu,et al. An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2023,120(25):e2207210120.
APA	Kuo Liu.,Muxue Tang.,Wei Xu.,Xinfeng Meng.,Hengwei Jin.,...&Bin Zhou.(2023).An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,120(25),e2207210120.
MLA	Kuo Liu,et al."An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 120.25(2023):e2207210120.